Description

6-TAMRA (CAS No. 150322-06-8) is a high-purity, fluorescent dye derivative of tetramethylrhodamine, renowned for its bright orange-red emission and excellent photostability. This compound is a critical reagent for labeling biomolecules, enabling sensitive detection and analysis in advanced life science research. It is essential for researchers and manufacturers in the fields of molecular diagnostics, fluorescence microscopy, and next-generation sequencing.

Application

• Oligonucleotide Labeling: Primarily used as an active ester (6-TAMRA SE) for covalently tagging primers, probes, and other synthetic oligonucleotides for real-time PCR (qPCR) and FISH applications.
• Protein & Peptide Conjugation: Serves as a fluorescent marker for tracking protein localization, interactions, and dynamics in cellular imaging and flow cytometry.
• Fluorescence Microscopy: A key fluorophore for immunostaining and in situ hybridization, providing high-contrast, photostable signals for detailed cellular and tissue imaging.
• Capillary Electrophoresis & HPLC: Used as a fluorescent tag for the sensitive detection and quantification of biomolecules during separation and analysis.
• Diagnostic Assay Development: Integral to the design and production of fluorescent lateral flow assays and other point-of-care diagnostic tools.
• Chemical Biology Probes: Employed in the synthesis of activity-based probes and sensors for studying enzymatic activity and metabolic pathways.

Basic Information

Product Name	6-TAMRA
CAS No.	150322-06-8
Molecular Formula	C25H22N2O5
Molecular Weight	430.46 g/mol
Synonyms	6-Carboxytetramethylrhodamine; 6-TAMRA Acid; 6-Carboxy-N,N,N',N'-tetramethylrhodamine; 6-Carboxy-TAMRA; 5(6)-TAMRA; Tetramethylrhodamine-6-carboxylic acid; Rhodamine 6G carboxylic acid; TAMRA 6-isomer
EINECS	Contact for details

Quality Control

Our 6-TAMRA is manufactured under strict quality management systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting critical quality attributes. We support compliance with relevant guidelines for use in regulated environments.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. The product is light-sensitive and should be handled under appropriate conditions to prevent degradation. For long-term storage, desiccate at -20°C.

Specification

Item	Specification
Appearance	Dark red to purple powder
Identification (HPLC/UV-Vis)	Conforms
Purity (HPLC)	≥ 95%
Absorption (λmax)	~555 nm
Emission (λmax)	~580 nm
Extinction Coefficient (ε)	~90,000 cm⁻¹M⁻¹
Loss on Drying	≤ 2.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.