Description

5'-O-(4,4-Dimethoxytrityl)-2'-O-[(tert-Butyl)Dimethylsilyl]-N-[[4-(tert-Butyl)Phenoxy]Acetyl]Guanosine-3'-(2-Cyanoethyl-N,N-Diisopropyl)Phosphoramidite is a highly specialized, protected phosphoramidite monomer essential for the solid-phase synthesis of oligonucleotides. This compound is critical for ensuring high coupling efficiency and sequence fidelity in the automated production of DNA and RNA strands. It is primarily required by pharmaceutical R&D teams, diagnostic kit manufacturers, and academic research institutions engaged in antisense therapy, siRNA development, and molecular diagnostics.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key building block (monomer) for the automated, machine-driven synthesis of custom DNA and RNA sequences.
• Therapeutic Oligonucleotide Manufacturing: For the production of active pharmaceutical ingredients (APIs) used in antisense oligonucleotides (ASOs), small interfering RNA (siRNA), and aptamers.
• Diagnostic Probe Development: Enables the creation of specific, high-affinity probes for PCR assays, microarrays, fluorescence in situ hybridization (FISH), and next-generation sequencing (NGS).
• Research-Grade Oligo Synthesis: Used in academic and industrial laboratories for synthesizing primers, linkers, and gene fragments for molecular biology and genetic engineering.
• Synthesis of Modified Oligonucleotides: Facilitates the incorporation of guanosine residues with specific backbone or base modifications to enhance stability, binding affinity, or therapeutic properties.
• Scale-Up and cGMP Production: Suitable for process development and current Good Manufacturing Practice (cGMP) compliant manufacturing of oligonucleotides for clinical trials and commercial therapeutics.

Basic Information

Product Name	5'-O-(4,4-Dimethoxytrityl)-2'-O-[(tert-Butyl)Dimethylsilyl]-N-[[4-(tert-Butyl)Phenoxy]Acetyl]Guanosine-3'-(2-Cyanoethyl-N,N-Diisopropyl)Phosphoramidite
CAS No.	149989-68-4
Molecular Formula	C58H75N6O9PSi
Molecular Weight	1059.30 g/mol
Synonyms	Guanosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-O-[[(1,1-dimethylethyl)dimethylsilyl]-N-[4-(1,1-dimethylethyl)phenoxy]acetyl]-3'-[2-cyanoethoxy(diisopropylamino)phosphinyl]-; DMTr-TBDMS-G(Pac)-CE Phosphoramidite; N-Pac-G(DMTr-TBDMS)-CE Phosphoramidite; 2'-O-TBDMS, N-Pac protected Guanosine Phosphoramidite; G Phosphoramidite (2'-O-TBDMS, N-Pac, 5'-O-DMTr); 5'-O-(4,4'-Dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)-N-[4-(tert-butyl)phenoxyacetyl]guanosine-3'-O-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite
EINECS	Contact for details

Quality Control

Every batch of our phosphoramidite monomers is produced and tested under strict quality management systems to ensure the high purity and consistency required for sensitive oligonucleotide synthesis. Quality is verified through comprehensive analytical methods including HPLC, 31P NMR, and 1H NMR to confirm identity, purity, and absence of critical impurities. A detailed Certificate of Analysis (COA) is provided with each shipment, and we support customer audits and regulatory submissions for therapeutic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) in a dedicated freezer. This product is hygroscopic (moisture-sensitive) and must be handled in a dry environment. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Retention time corresponds to reference standard
Identification (31P NMR)	Spectrum corresponds to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.