Description

n-Acetyl-Ile-Glu-Ala-Arg-p-Nitroanilide Acetate is a specialized chromogenic peptide substrate designed for the selective detection and quantification of specific protease activity. This compound matters for its high sensitivity and specificity in enzymatic assays, providing critical data for biochemical research and diagnostic development. It is primarily needed by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic industries for studying enzyme kinetics, screening inhibitors, and developing clinical test kits.

Application

• Protease Activity Assays: As a chromogenic substrate for the specific detection and kinetic analysis of target proteases, including thrombin and related serine proteases.
• Biochemical Research: Used in fundamental research to study enzyme mechanisms, inhibition profiles, and substrate specificity in controlled laboratory settings.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize potential therapeutic protease inhibitors for drug discovery programs.
• Diagnostic Development: A key component in the formulation of in-vitro diagnostic (IVD) test kits for clinical measurement of coagulation factors and other relevant enzymatic markers.
• Quality Control in Biologics: Used to monitor and ensure the consistency and activity of enzyme-based biopharmaceutical products during manufacturing.

Basic Information

Product Name	n-Acetyl-Ile-Glu-Ala-Arg-p-Nitroanilide Acetate
CAS No.	132567-19-2
Molecular Formula	C31H48N10O11 • C2H4O2
Molecular Weight	Contact for details
Synonyms	Ac-Ile-Glu-Ala-Arg-pNA Acetate; Chromogenic Substrate S-2238; H-D-Ile-Glu-Ala-Arg-pNA • AcOH; Thrombin Substrate I; Ac-Ile-Glu(OR)-Gly-Arg-pNA; Benzoyl-Ile-Glu-Gly-Arg-p-nitroanilide acetate; Bz-Ile-Glu-Gly-Arg-pNA Acetate; S2238
EINECS	Contact for details

Quality Control

Our n-Acetyl-Ile-Glu-Ala-Arg-p-Nitroanilide Acetate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and related substances is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. Due to its hygroscopic nature, allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake. For long-term storage, consider desiccant use.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.