Description

3'-Anthraniloyl-2'-Deoxy-Atp CAS NO 130996-25-7 is a specialized, fluorescently-labeled nucleotide analog designed for advanced biochemical research. This compound is critical for studying enzyme kinetics, particularly for kinases and ATPases, due to its ability to act as a substrate while providing a sensitive fluorescent signal. It is an essential tool for researchers and developers in pharmaceutical discovery, enzymology, and molecular diagnostics who require precise, real-time monitoring of enzymatic activity.

Application

• Enzyme Kinetics & High-Throughput Screening (HTS): Acts as a fluorescent substrate for continuous, homogeneous assays to measure the activity of kinases, ATPases, and other ATP-utilizing enzymes.
• Drug Discovery & Development: Used in target validation and inhibitor screening campaigns to identify and characterize novel therapeutic compounds.
• Biochemical Research: Serves as a vital probe for mechanistic studies of nucleotide-binding proteins and energy transduction pathways.
• Diagnostic Assay Development: Employed in the design of sensitive fluorescence-based detection systems for clinical and research diagnostics.
• Cellular Signaling Studies: Useful for investigating intracellular ATP-dependent processes when used with appropriate delivery systems.

Basic Information

Product Name	3'-Anthraniloyl-2'-Deoxy-Atp
CAS No.	130996-25-7
Molecular Formula	C22H27N6O13P3
Molecular Weight	700.42 g/mol
Synonyms	2'-Deoxy-3'-O-anthraniloyladenosine-5'-triphosphate; 3'-Anthraniloyl-2'-deoxyadenosine-5'-triphosphate; 2'-d-3'-ANT-ATP; Anthraniloyl-dATP; Fluorescent dATP analog; Mant-2'-dATP; 3'-O-(N-Methylanthraniloyl)-2'-deoxyadenosine-5'-triphosphate (common naming variant)
EINECS	Contact for details

Quality Control

Every batch of 3'-Anthraniloyl-2'-Deoxy-Atp is manufactured and tested to meet stringent quality standards suitable for sensitive research applications. We employ advanced analytical techniques, including HPLC and mass spectrometry, to ensure high purity and batch-to-batch consistency. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profiles is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store desiccated under inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (Karl Fischer)	≤ 3.0%
Fluorescence Properties	Excitation ~360 nm, Emission ~440 nm (verify in buffer)
Biological Activity	Functional as a substrate for representative kinase (e.g., PKA)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.