Description

Spacer 18 Phosphoramidite CAS NO 125607-09-2 is a high-purity, specialized phosphoramidite monomer used as a critical building block in the automated synthesis of oligonucleotides. This compound is essential for introducing a defined, 18-atom hexaethyleneglycol spacer arm into synthetic DNA or RNA strands, enabling precise control over molecular architecture. It is primarily required by biotechnology, pharmaceutical, and diagnostic companies engaged in advanced research, therapeutic development, and the production of probes and primers.

Application

• Oligonucleotide Synthesis: Key monomer for introducing long, flexible spacer (C18) linkages in solid-phase DNA/RNA synthesizers.
• Therapeutic Oligonucleotide Development: Used in the manufacture of antisense oligonucleotides, siRNA, and aptamers requiring specific spatial separation of functional groups.
• Diagnostic Probe Design: Incorporation into fluorescent or quencher-labeled probes (e.g., Molecular Beacons, TaqMan probes) to optimize signal-to-noise ratios.
• Microarray and Chip Fabrication: Synthesis of capture probes with controlled surface attachment and reduced steric hindrance.
• Bioconjugation and Immobilization: Creating linker arms for attaching oligonucleotides to surfaces, particles, or other biomolecules.
• Structural Studies: Building oligonucleotides with defined flexibility for NMR and crystallography research.

Basic Information

Item	Detail
Product Name	Spacer 18 Phosphoramidite
CAS No.	125607-09-2
Molecular Formula	C39H71N2O8P
Molecular Weight	727.0 g/mol
Synonyms	18-O-Dimethoxytritylhexaethyleneglycol,1-[(2-cyanoethoxy)(diisopropylamino)phosphino]-2-O-succinoyl-3-O-(4,4'-dimethoxytrityl)-; Hexaethyleneglycol Spacer Phosphoramidite; Spacer C18 Phosphoramidite; DMT-Hexaethyleneglycol Phosphoramidite; 18-Atom Spacer Phosphoramidite; 1-O-Dimethoxytrityl-hexaethyleneglycol-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; Spacer Phosphoramidite 18
EINECS	Contact for details

Quality Control

Our Spacer 18 Phosphoramidite is manufactured under strict quality systems to ensure batch-to-batch consistency for sensitive oligonucleotide synthesis. Each lot undergoes comprehensive analytical testing, including HPLC purity assessment, NMR confirmation of structure, and moisture content analysis. Certificates of Analysis (COA) with full specification data are provided and we support compliance with relevant GMP guidelines for advanced therapeutic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. Due to its hygroscopic and moisture-sensitive nature, allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in anhydrous acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.