Description

n-Blocked-5'-o-Dmt-2'-o-Tbdms Ced Adenosine Phosphoramidite is a highly specialized, protected nucleoside phosphoramidite monomer essential for solid-phase oligonucleotide synthesis. This compound is critical for ensuring high coupling efficiency and sequence fidelity in the automated production of DNA and RNA strands. It is primarily required by biotechnology and pharmaceutical companies engaged in the research, development, and manufacturing of synthetic oligonucleotides for therapeutic, diagnostic, and research applications.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key building block (monomer) in automated DNA/RNA synthesizers for the stepwise assembly of oligonucleotides.
• Therapeutic Oligonucleotide Manufacturing: Production of antisense oligonucleotides (ASOs), siRNA, aptamers, and other nucleic acid therapeutics.
• Diagnostic Probe Synthesis: Manufacture of labeled probes for PCR, qPCR, FISH, and microarray technologies.
• Research-Grade Oligo Production: Synthesis of primers, linkers, and gene fragments for molecular biology and genetic engineering research.
• CRISPR Guide RNA Synthesis: Component in the chemical synthesis of custom guide RNAs (gRNAs) for gene editing applications.
• Antisense Drug Development: Critical raw material in the R&D and scale-up phases of novel antisense-based pharmaceuticals.

Basic Information

Product Name	n-Blocked-5'-o-Dmt-2'-o-Tbdms Ced Adenosine Phosphoramidite
CAS No.	121058-86-4
Molecular Formula	C47H63N6O7PSi
Molecular Weight	883.10 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)-N-benzoyladenosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; N-Bz-5'-O-DMT-2'-O-TBDMS Adenosine-3'-CE Phosphoramidite; 2'-O-TBDMS, 5'-O-DMT Adenosine Phosphoramidite; Ced Phosphoramidite of Protected Adenosine; DMT-TBDMS-A(bz)-CE Phosphoramidite; DNA Synthesis Phosphoramidite Monomer (A); Amidite A (DMT, TBDMS, Bz, CE)
EINECS	Contact for details

Quality Control

Every batch of our nucleoside phosphoramidites is produced and tested under strict quality management systems to ensure the high purity and consistency required for sensitive oligonucleotide synthesis. Quality is verified through comprehensive analytical methods including HPLC, 31P NMR, and 1H NMR. A Certificate of Analysis (COA) detailing purity, moisture content, and performance in coupling tests is provided with each shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air, moisture, or elevated temperatures will lead to decomposition and reduced coupling efficiency. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Moisture Content (KF)	≤ 0.5%
Coupling Efficiency Test	≥ 99.0% per step

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.