Description

O(2',3')-(2,4,6-Trinitrophenyl)-8-Azidoadenosine Triphosphate is a specialized, chemically modified nucleotide analog designed for advanced biochemical research and labeling applications. This compound is critical for researchers requiring a high-affinity, photoactivatable probe for studying nucleotide-binding proteins, enzyme mechanisms, and nucleic acid interactions. It is primarily utilized by biochemists, molecular biologists, and pharmaceutical R&D teams in academic, diagnostic, and drug discovery laboratories.

Application

• Photoaffinity Labeling: Acts as a critical cross-linking agent for identifying and characterizing ATP-binding sites in proteins and enzymes.
• Enzyme Kinetics & Inhibition Studies: Used as a substrate analog to investigate the catalytic mechanisms of kinases, ATPases, and other nucleotide-dependent enzymes.
• Bioconjugation & Probe Synthesis: Serves as a key building block for creating fluorescent or affinity-tagged nucleotide probes via click chemistry (utilizing the azido group).
• Structural Biology: Aids in X-ray crystallography and NMR studies by providing a heavy-atom derivative (trinitrophenyl group) for phasing.
• Diagnostic Assay Development: Employed in the design of sensitive assays for detecting ATPase activity or nucleotide-protein interactions.
• Virology & Cell Signaling Research: Used to study viral polymerases and key signaling pathways involving nucleotide triphosphates.

Basic Information

Product Name	O(2',3')-(2,4,6-Trinitrophenyl)-8-Azidoadenosine Triphosphate
CAS No.	120218-67-9
Molecular Formula	C17H16N10O16P3
Molecular Weight	742.33 g/mol
Synonyms	8-Azido-2',3'-O-(2,4,6-trinitrophenyl)adenosine 5'-triphosphate; 8-N3-TNP-ATP; TNP-8-N3-ATP; 8-Azido-TNP-ATP; 8-Azidoadenosine-2',3'-O-(2,4,6-trinitrophenyl)triphosphate; Photoactivatable TNP-ATP; 8-Azido-2',3'-O-(2,4,6-trinitrophenyl)adenosine-5'-triphosphate
EINECS	Contact for details

Quality Control

Our production of O(2',3')-(2,4,6-Trinitrophenyl)-8-Azidoadenosine Triphosphate adheres to stringent quality protocols to ensure batch-to-batch consistency and research-grade purity. Each lot is characterized by multiple analytical techniques, including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and stability is provided with every shipment to support your critical research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is strictly light-sensitive (protect from all light exposure) and hygroscopic (moisture-sensitive). For long-term stability, store under inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (Karl Fischer)	≤ 1.0%
Solubility	Soluble in DMSO, aqueous buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.