Description

2'-Deoxy-5'-Adenylyl Imidodiphosphate is a specialized nucleotide analog that serves as a non-hydrolyzable ATP substitute. This compound is critical for researchers studying ATP-dependent enzymes and signaling pathways, as it binds to active sites but resists cleavage, effectively trapping enzyme-substrate complexes. It is an essential biochemical tool for scientists in molecular biology, enzymology, and structural biology who require precise mechanistic insights.

Application

• Enzyme Kinetics and Mechanism Studies: Used as a stable, non-hydrolyzable analog of ATP to investigate the catalytic mechanisms of kinases, ATPases, helicases, and other ATP-binding proteins.
• Structural Biology (X-ray Crystallography & Cryo-EM): Essential for co-crystallization experiments to determine high-resolution structures of protein-ligand complexes in their active states.
• Biochemical Assay Development: Serves as a key component in developing robust, high-throughput screening assays for drug discovery targeting ATP-binding sites.
• Signal Transduction Research: Employed to study and modulate ATP-dependent processes in cell signaling, metabolism, and nucleic acid remodeling.
• Control Experiments: Acts as a critical negative control in experiments to confirm the ATP-dependency of a reaction or to inhibit specific enzymatic activity.

Basic Information

Product Name	2'-Deoxy-5'-Adenylyl Imidodiphosphate
CAS No.	119447-19-7
Molecular Formula	C10H15N6O10P3
Molecular Weight	496.17 g/mol
Synonyms	Ado-5'-PP[NH]P; Adenylyl Imidodiphosphate, 2'-Deoxy-; 2'-Deoxyadenosine-5'-[β,γ-imido]triphosphate; 2'-d-AMP-PNP; 2'-Deoxy-AMP-PNP; Deoxyadenylyl imidodiphosphate; Non-hydrolyzable ATP analog; d-AMP-PNP; d-ADP(NH)P
EINECS	Contact for details

Quality Control

Our 2'-Deoxy-5'-Adenylyl Imidodiphosphate is produced under stringent conditions to ensure the high purity and consistency required for sensitive research applications. Each batch undergoes comprehensive analytical characterization, including HPLC, NMR, and mass spectrometry, to verify identity, purity, and the absence of critical contaminants like ATP or ADP. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all relevant specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccant and under an inert atmosphere to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Biological Activity Test	Suitable for use (Inhibition of ATPase)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.