Description

5'-o-Triphosphoryl-2-Azidoadenylyl-(2'-5')-2-Azidoadenylyl-(2'-5')-2-Azidoadenosine is a specialized, chemically modified trinucleotide analog designed for advanced biochemical research and development. This compound is critical for studying and manipulating RNA structure and function, particularly in the context of non-canonical 2'-5' linkages. It is an essential reagent for academic institutions, pharmaceutical R&D departments, and biotechnology companies focused on nucleic acid chemistry, antiviral drug discovery, and the development of novel molecular probes and diagnostics.

Application

• Biochemical Research: Used as a substrate or inhibitor in enzymatic studies of 2'-5'-oligoadenylate synthetase and related nucleotidyltransferases.
• Antiviral Mechanism Studies: Investigation of the 2-5A (2'-5'-oligoadenylate) pathway and its role in the innate immune response against viral infections.
• Probe Development: Acts as a key building block for creating photoaffinity labeling probes or cross-linking agents due to the presence of azido groups.
• RNA Analog Synthesis: Serves as a precursor or intermediate in the chemical synthesis of non-standard RNA oligonucleotides with 2'-5' phosphodiester bonds.
• Enzyme Characterization: Tool for characterizing the specificity and activity of nucleases, polymerases, and other nucleic acid-modifying enzymes.
• Drug Discovery Screening: Utilized in high-throughput assays to identify compounds that modulate pathways involving 2'-5'-linked oligoadenylates.

Basic Information

Item	Details
Product Name	5'-o-Triphosphoryl-2-Azidoadenylyl-(2'-5')-2-Azidoadenylyl-(2'-5')-2-Azidoadenosine
CAS No.	117146-00-6
Molecular Formula	C30H35N15O22P3
Molecular Weight	1058.62 g/mol
Synonyms	2-Azido-2'-5' oligoadenylate triphosphate; P1,P3-Bis(5'-adenosyl) Triphosphate, 2-azido derivative; 2-5A azido analog; 2'-5'-Oligo(2-azidoadenylic acid) triphosphate; 2-Azido-2',5'-oligoadenylate; Azido-2-5A; 5'-O-{[(2-Azidoadenylyl)-(2'->5')-(2-azidoadenylyl)-(2'->5')-2-azidoadenosine]}triphosphate
EINECS	Contact for details

Quality Control

Our production of this high-purity biochemical adheres to stringent quality protocols. Each batch is characterized using advanced analytical techniques including HPLC, mass spectrometry (MS), and NMR to confirm identity, purity, and structural integrity. A comprehensive Certificate of Analysis (COA) detailing assay purity, impurity profile, and solvent content is provided with every shipment to ensure traceability and support your critical research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (Karl Fischer)	≤ 2.0%
Solvent Residues (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.