Description

Beta,Beta'-Monochloromethylene Diadenosine 5',5'''-P(1),P(4)-Tetraphosphate is a specialized, high-purity nucleotide analog derivative, primarily utilized as a critical biochemical tool in advanced life science research. This compound matters for its role as a potent and specific inhibitor of certain ATP-dependent enzymes and as a non-hydrolyzable analog of Ap4A (diadenosine tetraphosphate), enabling detailed mechanistic studies of nucleotide signaling and energy metabolism pathways. It is essential for researchers in enzymology, molecular biology, and pharmacology who require precise chemical probes to investigate cellular energy transfer, signal transduction, and enzyme kinetics.

Application

• Enzyme Inhibition Studies: Used as a potent, non-hydrolyzable inhibitor for ATP-dependent enzymes such as kinases, ATPases, and aminoacyl-tRNA synthetases to elucidate reaction mechanisms.
• Nucleotide Signaling Research: Serves as a stable analog of diadenosine polyphosphates (ApnA) to study their roles in cellular stress response, apoptosis, and signal transduction pathways.
• Biochemical Assay Development: Employed in the development and validation of high-throughput screening assays for drug discovery targeting nucleotide-binding proteins.
• Structural Biology: Utilized in X-ray crystallography and NMR studies to determine the three-dimensional structure of enzyme-ligand complexes.
• Metabolic Pathway Analysis: Acts as a chemical tool to probe and modulate purinergic signaling and energy charge-related metabolic networks in cell-based studies.
• Pharmaceutical R&D: Investigated for potential therapeutic applications targeting diseases related to aberrant nucleotide signaling or energy metabolism.

Basic Information

Product Name	Beta,Beta'-Monochloromethylene Diadenosine 5',5'''-P(1),P(4)-Tetraphosphate
CAS No.	116371-34-7
Molecular Formula	C21H28ClN10O19P4
Molecular Weight	923.80 g/mol
Synonyms	P1,P4-Di(adenosine-5') tetraphosphate, beta,beta'-monochloromethylene analog; Adenosine-5'-[P1-(5'-adenosyl)]-beta,beta'-chloromethylenetetraphosphonate; beta,beta'-CM-Ap4A; beta,beta'-Chloromethylene-bis(adenosine-5'-tetraphosphate); Non-hydrolyzable Ap4A analog; Diadenosine 5',5'''-P1,P4-tetraphosphate, beta,beta'-monochloromethylene derivative; ARL 67156 (research code); ATP analog, Ap4A analog
EINECS	Contact for details

Quality Control

Our Beta,Beta'-Monochloromethylene Diadenosine 5',5'''-P(1),P(4)-Tetraphosphate is manufactured under strict quality management systems to ensure the highest standards of purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing including HPLC for purity, NMR for structural confirmation, and mass spectrometry for identity verification. Certificates of Analysis (COA) detailing all specifications are provided with every shipment. We adhere to ISO 9001 guidelines, and our processes are designed to meet the rigorous demands of pharmaceutical and academic research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep under an inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.