Description

5(6)-Carboxynaphthofluorescein,n-Succinimidylester is a reactive, amine-reactive fluorescent labeling reagent. This compound is crucial for creating stable, fluorescent conjugates with proteins, peptides, and other biomolecules containing primary amines. It is primarily needed by researchers and manufacturers in the life sciences, diagnostics, and biotechnology sectors for applications in fluorescence microscopy, flow cytometry, and immunoassay development.

Application

• Fluorescent Labeling of Proteins and Antibodies: Conjugation to primary amine groups (e.g., lysine residues) for use in immunofluorescence (IF), Western blotting, and ELISA.
• Biomolecule Tagging for Microscopy: Preparation of fluorescent probes for cellular and tissue imaging, including confocal and super-resolution microscopy.
• Flow Cytometry Reagent Synthesis: Creation of antibody-dye conjugates for cell surface marker detection and intracellular staining.
• Diagnostic Assay Development: Used in the manufacture of fluorescent detection components for clinical and research diagnostic kits.
• Bioconjugation and Chemical Biology: A key reagent for site-specific labeling strategies in proteomics and drug discovery research.
• Sensor and Probe Fabrication: Incorporation into polymeric or nanoparticle-based systems for environmental or biochemical sensing.

Basic Information

Product Name	5(6)-Carboxynaphthofluorescein,n-Succinimidylester
CAS No.	115034-75-8
Molecular Formula	C34H19NO9
Molecular Weight	585.52 g/mol
Synonyms	5(6)-Carboxy-2',7'-dichloro-3',6'-dihydroxy-4,5,6,7-tetrahydro-1H,3H-spiro[isobenzofuran-1,9'-xanthene]-3-one N-succinimidyl ester; 5(6)-Carboxy-2',7'-dichlorofluorescein N-succinimidyl ester; 5(6)-CDCF, SE; Naphthofluorescein isothiocyanate analogue, NHS ester; 5(6)-Carboxy-2',7'-dichloro-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3-one N-hydroxysuccinimide ester
EINECS	Contact for details

Quality Control

Our 5(6)-Carboxynaphthofluorescein,n-Succinimidylester is produced under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive bioconjugation applications. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Extinction Coefficient	Contact for details
Fluorescence Properties	Contact for details
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.