Description

Dt-Me Phosphonamidite CAS NO 114079-04-8 is a specialized organophosphorus compound, specifically a phosphoramidite monomer, which serves as a critical building block in oligonucleotide synthesis. Its primary value lies in enabling the precise, automated assembly of DNA and RNA strands, a cornerstone technology for modern life sciences. This high-purity reagent is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic industries developing therapeutics, probes, and primers.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated synthesis of DNA and RNA sequences.
• Therapeutic Oligonucleotide Production: Manufacturing of antisense oligonucleotides, siRNA, and aptamers for drug development.
• Research & Diagnostic Probe Synthesis: Creation of labeled probes for PCR, sequencing, fluorescence in situ hybridization (FISH), and microarray technologies.
• Primer Synthesis for Genomics: Production of high-fidelity primers used in next-generation sequencing (NGS) and genetic analysis.
• Synthesis of Modified Oligonucleotides: Incorporation into strands requiring specific backbone or terminal modifications for enhanced stability or functionality.
• Scale-Up cGMP Manufacturing: Sourcing for current Good Manufacturing Practice (cGMP) production of clinical-grade oligonucleotide APIs.

Basic Information

Product Name	Dt-Me Phosphonamidite
CAS No.	114079-04-8
Molecular Formula	C11H16N3O3P
Molecular Weight	269.24 g/mol
Synonyms	5'-O-Dimethoxytrityl-N2-isobutyryl-2'-deoxyguanosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; dG-Ibu Phosphoramidite (DMTr protected); Guanosine, 2'-deoxy-N2-(2-methyl-1-oxopropyl)-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-, 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]; Isobutyryl dG Phosphoramidite; 2'-Deoxy-N2-isobutyrylguanosine-5'-O-DMT-3'-O-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite
EINECS	Contact for details

Quality Control

Our Dt-Me Phosphonamidite is manufactured under strict quality controls to meet the exacting standards of oligonucleotide synthesis. Each batch is subjected to comprehensive analytical testing, including HPLC for purity and 31P NMR for structural confirmation, ensuring consistency and reliability for automated synthesizers. Certificates of Analysis (COA) with full traceability are provided for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C under an inert atmosphere (argon or nitrogen) due to its hygroscopic and moisture-sensitive nature. Allow the sealed container to reach ambient temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.5%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.