Description

1-[5-(o-Dimethoxytrityl)-2-Deoxy-β-D-Threo-Pentofuranosyl]Thymine is a high-purity, protected nucleoside derivative essential for oligonucleotide synthesis. This compound serves as a critical building block for the automated solid-phase synthesis of DNA and RNA sequences, ensuring high coupling efficiency and yield. It is primarily required by pharmaceutical R&D laboratories, biotechnology companies, and academic institutions engaged in the development of therapeutic oligonucleotides, antisense drugs, and diagnostic probes.

Application

• Oligonucleotide Synthesis: A key phosphoramidite precursor for the automated solid-phase synthesis of DNA and RNA strands.
• Therapeutic Oligonucleotide Development: Used in the research and production of antisense oligonucleotides, siRNA, and aptamers for targeted therapies.
• Diagnostic Probe Manufacturing: Essential for creating labeled probes used in PCR, FISH, and microarray technologies.
• Academic & Industrial Research: A fundamental reagent for studying nucleic acid chemistry, structure, and function.
• Scale-Up cGMP Production: Suitable for the manufacture of active pharmaceutical ingredients (APIs) for oligonucleotide-based drugs under controlled conditions.

Basic Information

Product Name	1-[5-(o-Dimethoxytrityl)-2-Deoxy-β-D-Threo-Pentofuranosyl]Thymine
CAS No.	112501-53-8
Molecular Formula	C31H32N2O7
Molecular Weight	544.60 g/mol
Synonyms	5'-O-DMT-2'-Deoxy-β-D-Threo-Pentofuranosyl Thymine; 5'-O-(4,4'-Dimethoxytrityl)-2'-deoxythymidine; DMT-dT; 5'-O-DMT-Thymidine; 1-[5-(4,4'-Dimethoxytrityl)-2-deoxy-β-D-threo-pentofuranosyl]-5-methyluracil; N-Thyminyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxyribose; Protected Thymidine; DMT-protected deoxythymidine
EINECS	Contact for details

Quality Control

Our 1-[5-(o-Dimethoxytrityl)-2-Deoxy-β-D-Threo-Pentofuranosyl]Thymine is manufactured under strict quality systems to meet the exacting standards of nucleic acid research and cGMP production. Each batch is subjected to rigorous analytical testing, including HPLC for purity and NMR for structural confirmation. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profiles is provided with every shipment to ensure full traceability and compliance.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to maintain stability and prevent decomposition.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, pyridine

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.