Description

Fluorescein Hydrazide is a highly reactive hydrazide derivative of the classic fluorescent dye, fluorescein. This compound is specifically engineered for the selective labeling and detection of aldehydes and ketones, forming stable, fluorescent conjugates. It is an essential reagent for researchers and manufacturers in life sciences, diagnostics, and materials science who require sensitive and specific detection methods.

Application

• Bioconjugation and Labeling: Primary application for tagging carbonyl groups (aldehydes, ketones) on proteins, antibodies, and other biomolecules for fluorescence detection.
• Glycan Analysis: Used in carbohydrate chemistry to label glycans and oligosaccharides for analysis via HPLC or capillary electrophoresis with fluorescence detection.
• Diagnostic Assay Development: Serves as a key fluorescent probe in the development of immunoassays, enzyme activity assays, and other diagnostic kits.
• Materials Science: Functionalization of polymers and surfaces that contain aldehyde or ketone moieties to introduce fluorescent properties for tracking or imaging.
• Cell Biology Research: Labeling of cellular components or metabolites that possess carbonyl groups for fluorescence microscopy and flow cytometry studies.
• Sensor Development: A critical component in the design of chemosensors and biosensors for detecting specific analytes that generate or contain carbonyl functionalities.

Basic Information

Product Name	Fluorescein Hydrazide
CAS No.	109653-47-6
Molecular Formula	C21H14N2O5
Molecular Weight	374.35 g/mol
Synonyms	Fluorescein 5(6)-Hydrazide; 5(6)-Fluorescein Hydrazide; Fluorescein Isomer I and II Hydrazide; 3',6'-Dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-carbohydrazide; 5- and 6-Isomers of Fluorescein Hydrazide; Fluorescein-5/6-hydrazide; F5/6H
EINECS	Contact for details

Quality Control

Our Fluorescein Hydrazide is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. A Certificate of Analysis (COA) detailing purity, identity, and other critical parameters is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and light-sensitive; ensure the container is sealed after each use to minimize exposure to atmospheric moisture and light.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0% (sum of isomers)
Water Content	≤ 5.0%
Fluorescence	Conforms (Ex/Em ~492/518 nm)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.