Description

25-Doxylcholesterol is a spin-labeled cholesterol analog, a vital tool compound in biophysical and biochemical research. Its primary value lies in its paramagnetic nitroxide radical, which serves as a sensitive probe for studying membrane dynamics, protein-lipid interactions, and sterol trafficking. This compound is essential for researchers and manufacturers in the pharmaceutical, life sciences, and advanced materials sectors who require precise molecular reporters for electron paramagnetic resonance (EPR) spectroscopy and related techniques.

Application

• As a spin probe in Electron Paramagnetic Resonance (EPR) spectroscopy to investigate membrane fluidity and phase transitions.
• Studying sterol distribution and trafficking within cellular membranes and lipid rafts.
• Research into protein-lipid interactions, particularly with membrane-bound receptors and transporters.
• Use as a molecular reporter in biophysical assays to model cholesterol behavior in synthetic lipid bilayers.
• Development and calibration of diagnostic and research kits for oxidative stress and membrane studies.
• Basic research in atherosclerosis and other diseases related to cholesterol metabolism.

Basic Information

Product Name	25-Doxylcholesterol
CAS No.	109024-15-9
Molecular Formula	C32H54NO3
Molecular Weight	500.78 g/mol
Synonyms	25-DOXYL-cholesterol; Cholestane-3β-ol, 25-(1-oxyl-2,2,6,6-tetramethyl-4-piperidinyl)-; 25-(4,4-Dimethyl-3-oxazolidinyloxy)cholest-5-en-3β-ol; 3β-Hydroxy-25-doxyl-5-cholestene; Cholesterol, 25-doxyl-; Spin-labeled cholesterol; EPR cholesterol analog
EINECS	Contact for details

Quality Control

Every batch of 25-Doxylcholesterol is produced and analyzed under strict quality management protocols to ensure high purity and consistent performance as a research-grade biochemical. We provide comprehensive Certificates of Analysis (COA) detailing purity, assay, and identity confirmation via techniques such as HPLC, NMR, and EPR spectroscopy. Our quality commitment supports reliable and reproducible experimental results for our global clientele in research and development.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C under an inert atmosphere (e.g., argon or nitrogen) to minimize oxidation of the nitroxide radical. Keep the container in a dry, cool, and well-ventilated area, separate from incompatible materials.

Specification

Item	Specification
Appearance	Off-white to pale yellow solid
Identification (HPLC)	Conforms
Identification (EPR)	Active nitroxide radical present
Assay (HPLC)	≥ 95.0%
Related Substances (HPLC)	Total impurities ≤ 5.0%
Solubility	Soluble in chloroform, ethanol, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.