Description

Pyrophosphohistidine is a specialized biochemical compound of significant interest in advanced research and development. This molecule plays a crucial role in studying enzyme mechanisms and signal transduction pathways, particularly those involving histidine phosphorylation. It is essential for researchers and manufacturers in the fields of pharmaceutical development, biochemical research, and diagnostic reagent formulation who require high-purity, well-characterized intermediates for their critical work.

Application

• Biochemical Research: Used as a key intermediate or reference standard in studies of histidine kinases, two-component signal transduction systems, and phosphohistidine biochemistry.
• Pharmaceutical Development: Serves as a precursor or tool compound in the discovery and development of novel therapeutics targeting bacterial signaling or specific metabolic pathways.
• Enzyme Mechanism Studies: Employed to investigate the kinetics and catalytic mechanisms of enzymes that utilize or modify phosphohistidine residues.
• Diagnostic Reagent Manufacturing: Acts as a critical component in the production of specialized assay kits and reagents for detecting specific enzymatic activities or biomarkers.
• Academic Research: Provides a valuable tool for universities and research institutions exploring protein phosphorylation beyond serine, threonine, and tyrosine.
• Metabolic Pathway Analysis: Used in metabolic studies to understand the role of histidine phosphorylation in cellular regulation and energy transfer.

Basic Information

Product Name	Pyrophosphohistidine
CAS No.	100764-00-9
Molecular Formula	C6H9N3O8P2
Molecular Weight	305.10 g/mol
Synonyms	1-(Diphosphonooxy)-L-histidine; L-Histidine, 1-(diphosphonooxy)-; Histidine pyrophosphate; 1-Pyrophosphoryl-L-histidine; Phosphoramidate derivative of histidine; PPH; His-PP
EINECS	Contact for details

Quality Control

Our Pyrophosphohistidine is produced under stringent quality management protocols to ensure batch-to-batch consistency and high purity for research applications. Each lot is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profiles is provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. Allow the sealed container to reach room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.