Description

Thymidine 5'-o-(1-Thiotriphosphate) is a chemically modified nucleotide analog where one of the non-bridging oxygen atoms in the triphosphate moiety is replaced by sulfur. This modification, specifically at the α-phosphate, makes it a crucial tool in molecular biology and biochemistry for studying enzyme mechanisms and creating nuclease-resistant nucleic acids. It is primarily utilized by researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic sectors for applications such as DNA sequencing, polymerase studies, and the synthesis of modified oligonucleotides.

Application

• Enzyme Kinetics & Mechanism Studies: Used as a substrate analog to investigate the catalytic mechanisms of DNA polymerases, reverse transcriptases, and other nucleotide-dependent enzymes.
• Site-Specific Labeling of Nucleic Acids: Enables the incorporation of a sulfur atom for subsequent chemical modification or to create phosphorothioate linkages, which confer nuclease resistance.
• Next-Generation Sequencing (NGS): Serves as a key reagent in certain sequencing-by-synthesis technologies where modified nucleotides are utilized.
• X-ray Crystallography: The sulfur atom can serve as a heavy atom for phasing in structural studies of enzyme-substrate complexes.
• Antisense & siRNA Research: Used in the synthesis of phosphorothioate oligonucleotides, which are more stable in biological fluids for therapeutic and research applications.
• Diagnostic Assay Development: Employed in the creation of specialized probes and primers for sensitive molecular detection systems.

Basic Information

Product Name	Thymidine 5'-o-(1-Thiotriphosphate)
CAS No.	18883-94-8
Molecular Formula	C10H17N2O13P3S
Molecular Weight	498.23 g/mol
Synonyms	dTTPαS; Thymidine 5'-(α-thiotriphosphate); 2'-Deoxythymidine 5'-(α-thiotriphosphate); 1-Thio-2'-deoxythymidine 5'-triphosphate; dTTP-α-S; α-Thio-dTTP; Adenosine 5'-(α-thiotriphosphate) (Note: This is a common related compound, often listed in searches); Deoxythymidine α-thiotriphosphate
EINECS	Contact for details

Quality Control

Our Thymidine 5'-o-(1-Thiotriphosphate) is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, suitable for sensitive research applications. Each lot is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. The product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Diastereomeric Purity	Contact for details
Biological Activity	Tested in enzymatic assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.