Description

Methylisopropylamiloride is a potent and selective inhibitor of the sodium-hydrogen exchanger (NHE), specifically targeting the NHE-1 isoform. This compound is a critical research tool for investigating cellular pH regulation, sodium ion transport, and their roles in cardiovascular and renal physiology. It is primarily utilized by pharmaceutical R&D teams, academic researchers, and contract research organizations (CROs) focused on ion channel biology, ischemia-reperfusion injury, and heart failure mechanisms.

Application

• Pharmacological Research: A key reference standard and probe for studying the structure-activity relationship (SAR) of amiloride analogs and NHE inhibitor efficacy.
• Cardiovascular Studies: Used in preclinical models to investigate myocardial protection, the role of NHE-1 in cardiac hypertrophy, and potential therapeutic interventions for ischemia.
• Cell Biology & Physiology: Essential for in vitro experiments examining intracellular pH (pHi) regulation, cell volume control, and sodium-dependent transport processes in various cell lines.
• Biochemical Assay Development: Serves as a control compound in high-throughput screening (HTS) campaigns to discover novel NHE inhibitors for drug discovery pipelines.
• Renal Function Research: Applied in studies of sodium reabsorption in renal tubules and the pathophysiology of conditions related to electrolyte imbalance.

Basic Information

Product Name	Methylisopropylamiloride
CAS No.	1151-74-2
Molecular Formula	C10H13Cl2N3O
Molecular Weight	254.14 g/mol
Synonyms	MIA; 3,5-Diamino-6-chloro-N-(diaminomethylene)-2-methyl-4-pyridinecarboxamide, N-isopropyl-N-methyl derivative; N'-Amino-N-(3,5-diamino-6-chloro-2-pyrazinyl)formimidamide, N-isopropyl-N-methyl derivative; Methylisopropyl Amiloride; N-Methyl-N-isopropylamiloride; 5-(N-Methyl-N-isopropyl)amiloride; EIPA analog
EINECS	Contact for details

Quality Control

Every batch of Methylisopropylamiloride is manufactured and tested under strict quality management systems. We provide comprehensive analytical data to ensure identity, purity, and consistency for research applications. A detailed Certificate of Analysis (COA) including HPLC purity, NMR confirmation, and residual solvent analysis is supplied with each shipment to meet the stringent requirements of the life sciences industry.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.