Description

n-(Deoxyguanosin-8-Yl)-2-Aminophenanthrene CAS NO 99504-05-9 is a specific DNA adduct formed by the covalent binding of a 2-aminophenanthrene metabolite to the C8 position of deoxyguanosine. This compound matters as a critical reference standard and biomarker in toxicology and cancer research, enabling the study of polycyclic aromatic hydrocarbon (PAH)-induced DNA damage and mutagenesis. It is primarily needed by research institutions, pharmaceutical R&D laboratories, and contract research organizations (CROs) focused on genetic toxicology, carcinogenesis mechanisms, and biomarker discovery.

Application

• Biomarker Standard for DNA Adduct Analysis: Used as a quantitative standard in mass spectrometry (LC-MS/MS) and 32P-postlabeling assays to detect and quantify specific DNA damage in biological samples.
• Mechanistic Toxicology Research: Essential for studying the metabolic activation pathways of 2-aminophenanthrene and the structural characterization of its DNA adducts to understand carcinogenic mechanisms.
• Reference Material in Genetic Toxicology: Serves as a positive control or calibration standard in assays designed to assess the genotoxic potential of PAHs and related compounds.
• Cancer Etiology Studies: Employed in research investigating the role of specific DNA lesions in the initiation and progression of cancers linked to PAH exposure.
• Method Development and Validation: Critical for developing and validating new analytical methods with high sensitivity and specificity for detecting low-abundance DNA adducts.
• Educational and Reference Standard: Used in academic settings for advanced teaching and research in chemical carcinogenesis and molecular toxicology.

Basic Information

Product Name	n-(Deoxyguanosin-8-Yl)-2-Aminophenanthrene
CAS No.	99504-05-9
Molecular Formula	C27H24N6O4
Molecular Weight	496.52 g/mol
Synonyms	N-(Deoxyguanosin-8-yl)-2-aminophenanthrene; 2-Aminophenanthrene-deoxyguanosine adduct; dG-C8-2-AP; 2-AP-DNA adduct; 8-(2-Aminophenanthren-1-yl)-2'-deoxyguanosine; N-(2'-Deoxyguanosin-8-yl)-2-aminophenanthrene; PAH-DNA Adduct Standard; 2-Aminophenanthrene Modified Deoxyguanosine
EINECS	Contact for details

Quality Control

This high-purity biochemical is manufactured under strict quality-controlled conditions. Each batch undergoes rigorous analytical characterization, including HPLC for purity assessment and mass spectrometric confirmation (MS, HRMS) of identity and structure. We ensure compliance with the exacting standards required for research-grade reference materials. A comprehensive Certificate of Analysis (COA) detailing purity, identity confirmation, and analytical data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dedicated freezer. This compound is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	Off-white to light tan solid
Identification (HPLC-MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.