Description

Fast Blue B Salt For Microscopy is a specialized diazonium salt primarily used as a coupling agent in histochemical staining. Its core value lies in producing a stable, insoluble blue azo dye when coupled with specific naphthol substrates, enabling precise visualization of enzymatic activity, particularly alkaline phosphatase, in tissue sections. This product is essential for researchers and diagnosticians in histopathology, neuroscience, and cell biology who require reliable and high-contrast staining for microscopic analysis.

Application

• Histochemical Staining: Primary coupling agent for the localization of alkaline phosphatase (ALP) activity in frozen or paraffin-embedded tissue sections.
• Neuroscience Research: Used in techniques to map neural pathways and identify specific neuronal populations.
• Diagnostic Pathology: Employed in clinical laboratories for enzyme-linked assays and in-situ hybridization protocols to aid disease diagnosis.
• Cell Biology Studies: Visualization of phosphatase activity in cultured cells for functional studies.
• Immunohistochemistry (IHC): Can be used as a chromogen in certain multiplex staining protocols.
• Botany and Plant Science: Detection of enzymatic activity in plant tissue samples.

Basic Information

Product Name	Fast Blue B Salt For Microscopy
CAS No.	14263-94-6
Molecular Formula	C₁₄H₁₄ClN₃O₃
Molecular Weight	307.73 g/mol
Synonyms	Fast Blue B; Diazo Blue B; Fast Blue BB Salt; 4'-Amino-2',5'-diethoxybenzanilide Diazonium Chloride Zinc Double Salt; 4-Benzoylamino-2,5-diethoxybenzenediazonium Chloride Zinc Chloride Double Salt; C.I. 37235; Azoic Diazo Component 20
EINECS	238-157-1

Quality Control

Our Fast Blue B Salt For Microscopy is manufactured and tested to ensure high performance in sensitive histological applications. Each batch undergoes rigorous quality testing, including assessment of coupling efficiency and purity, to guarantee consistent staining results. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and performance specifications is available for every lot upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This material is strictly light-sensitive and hygroscopic; prolonged exposure to light, moisture, or elevated temperatures will lead to decomposition and loss of activity. Keep container tightly sealed under desiccant when not in use.

Specification

Item	Specification
Appearance	Yellow to beige powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 95.0%
Solubility	Soluble in water, forms a clear to slightly hazy solution
Coupling Efficiency	Produces intense blue color with standard naphthol substrate
Loss on Drying	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.