Description

2,3-Diphenyl-5-(p-Tolyl)Tetrazolium Chloride is a specialized tetrazolium salt widely used as a vital reagent in biochemical and analytical applications. Its core value lies in its role as a redox indicator and a precursor for formazan dye formation, which is critical for cell viability and metabolic activity assays. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors.

Application

• Cell Viability and Proliferation Assays: A key component in MTT and related assays to measure mitochondrial activity in live cells.
• Microbiological Testing: Used as an indicator for bacterial growth and metabolic activity in microbiological media and environmental testing.
• Enzyme Activity Studies: Serves as an electron acceptor in dehydrogenase and reductase enzyme activity assays.
• Cytotoxicity Screening: Employed in high-throughput screening (HTS) platforms for drug discovery and toxicology studies.
• Biochemical Research: Utilized in various research applications involving redox reactions and electron transfer processes.
• Diagnostic Kits: Incorporated into commercial diagnostic kits for clinical and research use.

Basic Information

Product Name	2,3-Diphenyl-5-(p-Tolyl)Tetrazolium Chloride
CAS No.	10557-48-9
Molecular Formula	C26H21ClN4
Molecular Weight	424.92 g/mol
Synonyms	p-Tolyl Tetrazolium Chloride; TPTZ Chloride; 5-(4-Methylphenyl)-2,3-diphenyl-2H-tetrazolium Chloride; 2,3-Diphenyl-5-(4-methylphenyl)tetrazolium Chloride; Tetrazolium Red Chloride (related); MTT Tetrazolium Salt Analog; TTC Derivative; Cell Viability Assay Reagent
EINECS	Contact for details

Quality Control

Our 2,3-Diphenyl-5-(p-Tolyl)Tetrazolium Chloride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by IR and NMR spectroscopy, to ensure it meets the high standards required for sensitive biochemical applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept tightly sealed and desiccated after opening to maintain stability and performance.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Heavy Metals	≤ 20 ppm
Residue on Ignition	≤ 0.1%
Solubility	Soluble in methanol, DMSO; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.