Description

D-[2,5-13C2]Glucose is a stable isotope-labeled analog of D-glucose, specifically enriched with carbon-13 at the 2 and 5 positions. This compound is an essential tool for advanced metabolic research, enabling precise tracking and quantification of glucose utilization and flux through biochemical pathways. It is primarily utilized by researchers and analytical laboratories in the fields of metabolomics, pharmaceutical development, and clinical diagnostics to gain deeper insights into cellular energy metabolism and disease mechanisms.

Application

• Tracer Studies in Metabolic Flux Analysis (MFA): Used as a isotopic tracer to map and quantify intracellular metabolic pathways in cell cultures, tissues, and whole organisms.
• Drug Metabolism and Pharmacokinetics (DMPK): Employed in pharmaceutical R&D to study the metabolic fate of new drug candidates and their interaction with glucose metabolism.
• Clinical Research and Diagnostics: Facilitates investigations into metabolic disorders such as diabetes, cancer metabolism, and inborn errors of metabolism.
• NMR and Mass Spectrometry Standards: Serves as an internal standard or calibrant in quantitative analytical techniques like 13C-NMR and LC-MS/MS for accurate metabolite profiling.
• Biotechnology and Fermentation Monitoring: Applied in optimizing bioprocesses to track carbon source utilization and improve yield in microbial or cell-based production systems.

Basic Information

Product Name	D-[2,5-13C2]Glucose
CAS No.	86595-19-9
Molecular Formula	C6H12O6 (with 13C at positions 2 & 5)
Molecular Weight	182.15 g/mol (for 13C2 labeled form)
Synonyms	D-Glucose-2,5-13C2; (2,5-13C2)-D-Glucose; D-Glucose (2,5-13C2); 2,5-13C2-D-Glucose; D-(+)-Glucose-2,5-13C2; Isotopically Labeled D-Glucose; 13C2-Glucose; Stable Isotope-Labeled Glucose
EINECS	Contact for details

Quality Control

Every batch of D-[2,5-13C2]Glucose is manufactured and analyzed under strict quality protocols to ensure the highest standards of isotopic and chemical purity, critical for sensitive research applications. Our quality assurance includes comprehensive testing for isotopic enrichment, chemical purity, and identity. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting specifications such as isotopic purity (atom % 13C), chemical purity (by HPLC), and residual solvent content.

Storage

Preserve in a tightly closed container, protected from light. This product is hygroscopic (moisture-sensitive). For long-term stability, store at 2-8°C in a cool, dry place. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Isotopic Purity (atom % 13C)	≥ 99% (at positions 2 & 5)
Chemical Purity (HPLC)	≥ 98.0%
Specific Optical Rotation	+52.5° to +53.0° (c=10 in H2O)
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.