Description

4-Cholesten-3-One-3,4-13C2 is a stable isotope-labeled analog of cholestenone, specifically enriched with carbon-13 at the 3 and 4 positions. This high-purity labeled compound is essential for advanced analytical research, providing an internal standard for precise quantification and metabolic pathway tracing via mass spectrometry. It is primarily utilized by research institutions and pharmaceutical companies engaged in lipid metabolism studies, steroid biosynthesis research, and the development of diagnostic assays.

Application

• Internal Standard for Quantitative Analysis: Serves as a critical reference standard in LC-MS and GC-MS methods for the accurate measurement of endogenous cholestenone and related sterols in biological matrices.
• Metabolic Pathway Tracing: Used in isotopic labeling studies to elucidate biosynthetic pathways and metabolic flux of cholesterol and steroid hormones in in vitro and in vivo systems.
• Pharmaceutical Research & Development: Employed in drug discovery programs targeting lipid metabolism enzymes, aiding in the pharmacokinetic and pharmacodynamic evaluation of novel therapeutics.
• Clinical Diagnostics Development: Acts as a key component in the development and validation of high-sensitivity clinical mass spectrometry assays for detecting sterol disorders.
• Biochemical Research: A vital tool for studying enzyme kinetics, inhibitor screening, and mechanistic studies involving steroidogenic enzymes like HSD3B.
• Nutritional Science: Applied in research investigating the impact of diet and nutrients on cholesterol synthesis and catabolism.

Basic Information

Product Name	4-Cholesten-3-One-3,4-13C2
CAS No.	82938-09-8
Molecular Formula	C2713C2H44O
Molecular Weight	386.66 g/mol (for 13C2 labeled form)
Synonyms	Cholest-4-en-3-one-3,4-13C2; δ4-Cholesten-3-one-3,4-13C2; 3,4-13C2-Cholestenone; 4-Cholesten-3-one-3,4-13C2; Isotopically Labeled Cholestenone; [3,4-13C2]-4-Cholesten-3-one
EINECS	Contact for details

Quality Control

Every batch of 4-Cholesten-3-One-3,4-13C2 is manufactured and analyzed under strict quality management protocols. We guarantee high chemical and isotopic purity, with comprehensive testing including HPLC for chemical purity, NMR and MS for structural confirmation and isotopic enrichment verification. Certificates of Analysis (COA) detailing batch-specific results for identity, purity, and isotopic abundance are provided and available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure containers are sealed tightly after each use to minimize exposure to moisture and light.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 99 atom % 13C
Chemical Purity (by 1H NMR)	≥ 97.0%
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.