Description

n-(Deoxyguanosin-8-Yl)Benzidine is a specific DNA adduct formed from the covalent binding of benzidine metabolites to the C8 position of deoxyguanosine. This compound is of critical importance in toxicology and cancer research as a definitive biomarker of exposure to benzidine, a known human carcinogen. It is primarily needed by research institutions, analytical laboratories, and pharmaceutical companies engaged in genetic toxicology studies, biomonitoring, and the development of detection methods for carcinogen-DNA interactions.

Application

• Biomarker Research: Used as a reference standard for quantifying benzidine-DNA adducts in biological samples to assess carcinogen exposure and metabolic activation.
• Toxicological Studies: Essential for in vitro and in vivo studies investigating the mechanisms of benzidine-induced mutagenesis and carcinogenesis.
• Analytical Method Development: Serves as a critical calibrant in advanced analytical techniques like LC-MS/MS and HPLC for sensitive adduct detection.
• Cancer Risk Assessment: Employed in epidemiological research to establish dose-response relationships for benzidine exposure and cancer risk.
• Standards for Regulatory Testing: Used to validate assays for compliance with regulatory guidelines (e.g., OECD, ICH) for genotoxicity testing.
• DNA Repair Studies: Applied in research focused on understanding the efficiency and pathways of repair for specific bulky DNA adducts.

Basic Information

Product Name	n-(Deoxyguanosin-8-Yl)Benzidine
CAS No.	82682-91-5
Molecular Formula	C22H22N8O4
Molecular Weight	462.46 g/mol
Synonyms	N-(Deoxyguanosin-8-yl)-benzidine; 8-(Benzidin-N-yl)-2'-deoxyguanosine; Benzidine-deoxyguanosine adduct; dG-BZ; N-(Deoxyguanosin-8-yl)-4,4'-diaminobiphenyl; DNA-Benzidine Adduct; 8-(4-Aminobiphenyl-4-ylamino)-2'-deoxyguanosine
EINECS	Contact for details

Quality Control

Our n-(Deoxyguanosin-8-Yl)Benzidine is produced and handled under strict quality-controlled conditions to ensure the highest standard required for research applications. Each batch is characterized using advanced spectroscopic and chromatographic methods to confirm identity and purity. A comprehensive Certificate of Analysis (COA) detailing purity, identity confirmation (e.g., by NMR, MS), and chromatographic profile (HPLC) is provided with every shipment to guarantee traceability and reliability for your critical studies.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to light, heat, or humidity should be avoided to maintain stability. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Off-white to light tan solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.