Description

Indole-3-Acetic Acid, [5-3H] is a tritium-labeled derivative of the essential plant hormone auxin, indole-3-acetic acid (IAA). This high-specific-activity radiolabeled compound is a critical tool for tracing metabolic pathways, studying receptor binding, and quantifying hormone transport in plant physiology and biochemistry research. It is indispensable for researchers in agricultural biotechnology, plant molecular biology, and environmental science who require precise, sensitive detection methods.

Application

• Plant Hormone Metabolism Studies: Tracing the biosynthesis, conjugation, and catabolic pathways of auxin in plant tissues.
• Receptor Binding Assays: Quantifying the affinity and kinetics of auxin binding to its receptor proteins (e.g., TIR1/AFB).
• Polar Auxin Transport Research: Visualizing and measuring the directional flow of IAA within plants, crucial for understanding tropisms and development.
• Environmental Fate Analysis: Investigating the uptake, translocation, and degradation of synthetic auxin analogs or pollutants in plants.
• Autoradiography and Imaging: Enabling high-resolution spatial localization of auxin within specific cells and tissues.
• Enzyme Activity Assays: Serving as a substrate for studying the activity of IAA-amido synthetases (GH3) or oxidases.

Basic Information

Product Name	Indole-3-Acetic Acid, [5-3H]
CAS No.	82462-44-0
Molecular Formula	C10H9NO2 (3H)
Molecular Weight	~175.2 g/mol (varies with specific activity)
Synonyms	3-Indoleacetic acid-5-3H; [5-3H]IAA; Tritiated IAA; Auxin, tritium-labeled; 1H-Indole-3-acetic acid, 5-tritio-; (5-3H)Indol-3-ylacetic acid; Radioactive indoleacetic acid
EINECS	Contact for details

Quality Control

Every batch of Indole-3-Acetic Acid, [5-3H] is manufactured and analyzed under strict radiochemical purity and GMP-like guidelines. Quality is verified by HPLC with radiometric detection to ensure specified chemical and radiochemical purity, typically >97%. Certificates of Analysis (COA) detailing specific activity, concentration, solvent, and purity are provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at or below -20°C in the original solvent as supplied. Due to its sensitivity to light and oxidation, this material must be handled under an inert atmosphere when aliquoting and stored under conditions recommended for sensitive radiochemicals. Keep away from strong acids, bases, and oxidizing agents.

Specification

Item	Specification
Appearance	Clear solution (color may vary)
Concentration	Specific to batch (e.g., 1 mCi/mL)
Specific Activity	10-30 Ci/mmol (Contact for exact value)
Chemical Purity (HPLC)	≥ 97%
Radiochemical Purity (HPLC)	≥ 97%
Solvent	Ethanol:Water or custom (see COA)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.