Description

22,25-Dihydroxyvitamin D3 is a key metabolite in the vitamin D3 metabolic pathway, playing a significant role in calcium homeostasis and bone metabolism. This high-purity compound is essential for advanced pharmaceutical research and development, particularly in studying endocrine regulation and developing novel therapeutics. It is primarily utilized by research institutions, pharmaceutical manufacturers, and biotechnology companies focused on metabolic disorders, osteoporosis, and renal diseases.

Application

• Pharmaceutical Reference Standard: Serves as a certified reference material (CRM) for quality control and analytical method development in drug manufacturing.
• Biochemical Research: Used as a critical reagent in metabolic pathway studies, receptor binding assays, and enzymatic activity research related to vitamin D.
• Drug Discovery & Development: Acts as a key intermediate or active pharmaceutical ingredient (API) in the synthesis of novel vitamin D analogs for therapeutic applications.
• Clinical Diagnostics: Employed in the calibration and validation of diagnostic kits and LC-MS/MS methods for accurately measuring vitamin D metabolites in serum.
• Academic Research: Fundamental tool for in vitro and in vivo studies investigating the role of vitamin D in cell differentiation, immune function, and cancer research.

Basic Information

Product Name	22,25-Dihydroxyvitamin D3
CAS No.	81446-12-0
Molecular Formula	C27H44O3
Molecular Weight	416.64 g/mol
Synonyms	22,25-Dihydroxycholecalciferol; (5Z,7E)-9,10-Secocholesta-5,7,10(19)-triene-3β,22,25-triol; 22,25-Dihydroxyvitamin D3; 22,25-(OH)2D3; Vitamin D3 metabolite 22,25-diol
EINECS	Contact for details

Quality Control

Our 22,25-Dihydroxyvitamin D3 is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for research and pharmaceutical applications. A detailed Certificate of Analysis (COA) is provided with every shipment, guaranteeing traceability and consistent batch-to-batch quality.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to prevent oxidation. Allow the sealed container to reach room temperature before opening to minimize moisture condensation. For long-term storage, keep desiccated.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (IR)	Spectrum matches reference spectrum
Purity (HPLC)	≥ 98.0%
Related Substances (HPLC)	Total impurities ≤ 2.0%
Solvent Residues (GC)	Complies with ICH guidelines
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.