Description

23,24,25-Trihydroxyvitamin D3 is a biologically active metabolite of Vitamin D3, representing a key intermediate in the hormonal calcitriol pathway. This high-purity compound is critical for research and development in endocrinology and metabolic bone disease studies. It is primarily utilized by pharmaceutical R&D laboratories, academic research institutions, and manufacturers of advanced reference standards and diagnostic kits.

Application

• Pharmaceutical Research & Development: Serves as a crucial reference standard and intermediate in the study of Vitamin D metabolism, receptor binding, and the development of novel therapeutics for osteoporosis, renal disease, and psoriasis.
• Biochemical Assay Development: Used in the calibration and validation of competitive binding assays (CBAs), radioimmunoassays (RIAs), and liquid chromatography-mass spectrometry (LC-MS/MS) methods for accurate quantification of Vitamin D metabolites.
• Academic & Clinical Studies: Enables fundamental research into the non-classical actions of Vitamin D, including its roles in immune modulation, cell differentiation, and cancer biology.
• Reference Material Production: A vital raw material for certified reference material (CRM) manufacturers supplying quality control laboratories in the pharmaceutical and clinical diagnostics industries.
• Metabolic Pathway Analysis: Employed in tracer studies to elucidate the detailed enzymatic pathways and catabolism of Vitamin D3 in various biological systems.

Basic Information

Product Name	23,24,25-Trihydroxyvitamin D3
CAS No.	80463-20-3
Molecular Formula	C₂₇H₄₄O₅
Molecular Weight	448.64 g/mol
Synonyms	1α,25-Dihydroxy-23,24,25,26,27-pentanorvitamin D3-24-carboxylic acid; 23,24,25-Trihydroxycholecalciferol; (1R,3S)-5-[2-[(1R,3aS,7aR)-1-[(2R)-6-Hydroxy-6-methylheptan-2-yl]-7a-methyl-2,3,3a,5,6,7-hexahydro-1H-inden-4-ylidene]ethylidene]-4-methylidenecyclohexane-1,3-diol; Vitamin D3 metabolite 23,24,25-(OH)₃D₃
EINECS	Contact for details

Quality Control

Our 23,24,25-Trihydroxyvitamin D3 is manufactured under strict quality management systems. Each batch is subjected to comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods (NMR, MS), to ensure it meets the exacting standards required for research and development. Certificates of Analysis (COA) with detailed chromatographic data are provided for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. The product is hygroscopic (moisture-sensitive) and light-sensitive; allow the sealed vial to equilibrate to room temperature before opening to minimize moisture ingress.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in ethanol, DMSO; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.