Description

Spiperone, [Benzene Ring-3H] is a radiolabeled analog of the potent dopamine D2 and serotonin 5-HT2A receptor antagonist spiperone, specifically tritiated on the benzene ring. This labeled compound is an essential tool for high-sensitivity receptor binding assays and autoradiography studies in neuropharmacological research. It is primarily utilized by pharmaceutical R&D teams, academic neuroscientists, and contract research organizations (CROs) investigating the central nervous system.

Application

• Receptor Binding Assays: Quantitative analysis of dopamine D2 and serotonin 5-HT2A receptor density and affinity in brain tissue homogenates.
• Autoradiography: Spatial mapping and visualization of receptor distribution in brain slices for neuroanatomical studies.
• Drug Discovery Screening: High-throughput screening of novel compounds for antipsychotic or neuropsychiatric drug candidates targeting dopaminergic and serotonergic pathways.
• Pharmacological Research: Investigating receptor occupancy, kinetics, and the mechanism of action of psychotropic drugs.
• Metabolic Studies: Tracing metabolic pathways and stability of the spiperone molecule in in vitro systems (requires specific handling due to tritium).
• Quality Control Reference Standard: Serving as a calibrated reference material in bioanalytical laboratories.

Basic Information

Product Name	Spiperone, [Benzene Ring-3H]
CAS No.	80154-50-3
Molecular Formula	C23H26FN3O2 (3H)
Molecular Weight	~395.5 g/mol (varies with specific activity)
Synonyms	8-[4-(4-Fluorophenyl)-4-oxobutyl]-1-phenyl-1,3,8-triazaspiro[4.5]decan-4-one, [Benzene Ring-3H]; [3H]Spiperone; Spiperone, tritiated; Spiroperidol, [Benzene Ring-3H]; R 5147; Lederle 5147; NSC 113925
EINECS	Contact for details

Quality Control

Every batch of Spiperone, [Benzene Ring-3H] is manufactured and analyzed under strict quality systems. Radiochemical purity and specific activity are determined by validated methods including HPLC with radiometric detection. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing specific activity, purity, solvent, and analytical results to ensure reproducibility and reliability for your critical research.

Storage

Preserve in a tightly closed container, protected from light. Store at or below -20°C in the original solvent as supplied. Due to its highly volatile nature and the presence of tritium, store in a dedicated, well-ventilated area for radioactive materials. Follow all local regulations for handling and disposal of radioactive compounds.

Specification

Item	Specification
Appearance	Clear, colorless to slightly yellow solution
Identification (HPLC & MS)	Conforms to reference standard
Specific Activity	15-30 Ci/mmol (555-1110 GBq/mmol)
Radiochemical Purity (HPLC)	≥ 97%
Chemical Purity (HPLC)	≥ 98%
Solvent	Ethanol or Toluene/Ethanol
Concentration	1.0 mCi/mL (37 MBq/mL)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.