Description

L-Histidine-13C6,15N3 is a stable isotope-labeled form of the essential amino acid L-Histidine, where six carbon atoms and three nitrogen atoms are replaced with their heavier isotopes. This compound is critical for advanced research requiring precise metabolic and proteomic pathway tracing. It is primarily utilized by pharmaceutical R&D teams, academic research institutions, and diagnostic manufacturers engaged in mass spectrometry-based studies, clinical research, and the development of labeled internal standards.

Application

• Mass Spectrometry (MS) & Quantitative Proteomics: Serves as an essential internal standard for the accurate quantification of histidine and histidine-containing peptides/proteins.
• Metabolic Flux Analysis (MFA): Used to trace and model metabolic pathways in cell cultures, tissues, or whole organisms to study disease mechanisms or bioprocess optimization.
• Clinical Research & Diagnostics: Employed in the development of isotope dilution assays for the precise measurement of histidine levels in biological fluids for nutritional or metabolic disorder studies.
• Pharmaceutical Development: Integral in pharmacokinetic (PK) and pharmacodynamic (PD) studies to track the absorption, distribution, metabolism, and excretion (ADME) of drug candidates.
• NMR Spectroscopy: Acts as a valuable probe in nuclear magnetic resonance studies due to the distinct magnetic properties of 13C and 15N nuclei.
• Nutritional Science Research: Used in stable isotope feeding studies to investigate protein metabolism, amino acid requirements, and turnover rates.

Basic Information

Product Name	L-Histidine-13C6,15N3
CAS No.	741656-40-6
Molecular Formula	13C6H915N3O2
Molecular Weight	164.10 g/mol (for labeled form)
Synonyms	(2S)-2-Amino-3-(1H-imidazol-4-yl)propanoic-13C6,15N3 acid; Histidine-13C6,15N3; L-Histidine (U-13C6, 15N3); [U-13C6,15N3]-L-Histidine; Stable Isotope Labeled L-Histidine; Histidine Internal Standard; Histidine-IS
EINECS	Contact for details

Quality Control

Every batch of L-Histidine-13C6,15N3 is manufactured and analyzed under strict quality systems to ensure the highest isotopic and chemical purity for sensitive research applications. Our products undergo rigorous quality testing, including advanced spectroscopic and chromatographic methods, to ensure compliance with industry standards. Certificates of Analysis (COA) detailing isotopic enrichment, chemical purity, and analytical results are provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation or storage under inert atmosphere to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 99 atom % 13C; ≥ 99 atom % 15N
Optical Rotation	[α]D20: +11.5° to +13.5° (c=1 in H2O)
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.