Description

Cholesterol-3,4-13C2 is a stable isotope-labeled analog of cholesterol, specifically enriched with two carbon-13 atoms at the 3 and 4 positions of the steroid backbone. This precise labeling makes it an indispensable internal standard for quantitative mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy, ensuring unparalleled accuracy in metabolic and lipidomic research. It is primarily utilized by pharmaceutical R&D laboratories, clinical diagnostic developers, and advanced academic research institutions engaged in lipid metabolism studies, biomarker discovery, and drug development.

Application

• Quantitative Mass Spectrometry (LC-MS/MS, GC-MS): Serves as a critical internal standard for the precise quantification of cholesterol and its metabolites in complex biological matrices like plasma, serum, and tissues.
• Metabolic Flux Analysis: Used as a tracer to study cholesterol biosynthesis pathways, turnover rates, and metabolic disorders in cell culture and animal models.
• NMR Spectroscopy: Provides a distinct spectral signature for structural elucidation and dynamic studies of cholesterol-containing systems, such as lipid bilayers and lipoproteins.
• Clinical Diagnostics & Biomarker Validation: Essential for developing and validating high-precision clinical assays measuring cholesterol levels for cardiovascular disease risk assessment.
• Pharmaceutical R&D: Employed in pharmacokinetic and pharmacodynamic studies of lipid-lowering drugs to monitor drug efficacy and metabolic effects.
• Food & Nutritional Science: Used in research to accurately measure cholesterol content and study its absorption and metabolism in fortified foods and dietary studies.

Basic Information

Product Name	Cholesterol-3,4-13C2
CAS No.	78887-48-6
Molecular Formula	C2713C2H46O
Molecular Weight	~389.66 g/mol (for 13C2 labeled form)
Synonyms	(3β)-Cholest-5-en-3-ol-3,4-13C2; 5-Cholesten-3β-ol-3,4-13C2; [3,4-13C2]-Cholesterol; Cholesterol-13C2 (3,4); 13C-Labeled Cholesterol; Isotopic Cholesterol Standard; Stable Isotope-Labeled Cholesterol
EINECS	Contact for details

Quality Control

Every batch of Cholesterol-3,4-13C2 is manufactured and analyzed under strict quality systems. We provide comprehensive analytical data to ensure the product meets the high standards required for sensitive analytical applications. Key parameters include isotopic purity (chemical and isotopic), chromatographic purity, and confirmation of structure. A Certificate of Analysis (COA) detailing lot-specific results from NMR, HPLC, and MS is supplied with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C or as indicated on the label/Certificate of Analysis. For long-term storage, consider storage at -20°C under an inert atmosphere. Keep the container in a dry, cool, and well-ventilated place.

Specification

Item	Specification
Appearance	White to off-white crystalline solid
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (13C)	≥ 99 atom % 13C
Chemical Purity	≥ 98.0%
Solubility	Soluble in chloroform, ether, slightly soluble in alcohol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.