Description

L-Leucine-[3,4,5-3H(N)] is a high-specific-activity, tritium-labeled isotopologue of the essential amino acid L-Leucine. This compound is critical for advanced metabolic and biochemical research, enabling precise tracing and quantification of leucine uptake, protein synthesis, and amino acid metabolism in biological systems. It is an indispensable tool for researchers and scientists in the fields of pharmaceutical development, nutritional science, and metabolic disorder studies.

Application

• Biomedical Research: Used as a radiotracer in autoradiography and liquid scintillation counting to study protein turnover, muscle metabolism, and cellular signaling pathways.
• Drug Discovery & Development: Essential for pharmacokinetic (PK) and absorption, distribution, metabolism, and excretion (ADME) studies of new therapeutic compounds.
• Metabolic Pathway Analysis: Enables detailed investigation of branched-chain amino acid (BCAA) catabolism and its role in conditions like diabetes, obesity, and inborn errors of metabolism.
• Nutritional Science: Applied in research on dietary protein utilization, muscle protein synthesis, and the metabolic effects of leucine supplementation.
• Cell Culture & In Vitro Studies: Used to label proteins in cell lines for pulse-chase experiments and to monitor real-time metabolic flux.

Basic Information

Product Name	L-Leucine-[3,4,5-3H(N)]
CAS No.	78333-15-0
Molecular Formula	C6H103H3NO2 *
Molecular Weight	~136.16 g/mol (for unlabeled); Actual MW varies with specific activity.
Synonyms	(2S)-2-Amino-4-methyl-[3,4,5-3H]pentanoic acid; L-[3,4,5-3H]Leucine; Tritiated L-Leucine; [3H]-L-Leucine; Leucine, L-, [3,4,5-3H(N)]-; (S)-2-Amino-4-methylpentanoic-3,4,5-3H acid; H-Leu-OH (tritiated).
EINECS	Contact for details

* Formula indicates tritium (3H) labeling at the 3,4,5 positions of the carbon chain.

Quality Control

Every batch of L-Leucine-[3,4,5-3H(N)] is manufactured and analyzed under strict quality systems to ensure radiochemical purity, specific activity, and chemical identity. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) detailing specific activity (Ci/mmol or GBq/mmol), radiochemical purity (by HPLC), chemical purity, and solvent composition are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at or below -20°C in the original solvent as supplied. For long-term storage, keep at -70°C to minimize radiolytic decomposition. Follow all applicable local and international regulations for the safe handling and disposal of radioactive materials.

Specification

Item	Specification
Appearance	Clear, colorless to slightly yellow solution
Specific Activity	60 - 120 Ci/mmol (2.2 - 4.4 TBq/mmol) [Custom ranges available]
Radiochemical Purity (HPLC)	≥ 95%
Chemical Purity (HPLC)	≥ 98%
Identification (IR / NMR)	Conforms to structure
Solvent	Ethanol:Water mixture (specified on COA)
Concentration	1 mCi/mL (37 MBq/mL) [Custom concentrations available]

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.