Description

Thymidine, Methyl-D3 CAS NO 74848-84-3 is a deuterium-labeled isotopologue of thymidine, a fundamental nucleoside building block of DNA. This compound is specifically designed for use as an internal standard in quantitative mass spectrometry, providing unmatched accuracy and reliability in analytical workflows. It is an essential reagent for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic sectors who require precise quantification of nucleosides, metabolites, and related compounds.

Application

• LC-MS/MS Internal Standard: Primary application for the accurate quantification of unlabeled thymidine and its metabolites in biological matrices (plasma, urine, tissue).
• Metabolomics & Biomarker Discovery: Used in stable isotope dilution assays to study metabolic pathways, cellular turnover, and identify disease biomarkers.
• Pharmacokinetic (PK) & Toxicokinetic (TK) Studies: Critical for determining the absorption, distribution, metabolism, and excretion (ADME) profiles of nucleoside-based drugs.
• DNA Synthesis & Repair Research: Serves as a tracer in studies investigating DNA replication, repair mechanisms, and cell proliferation.
• Quality Control in Pharmaceutical Manufacturing: Ensures the purity and correct quantification of active pharmaceutical ingredients (APIs) in nucleoside analog drugs.
• Clinical Diagnostics: Applied in assays for monitoring disease states, such as cancers, where thymidine kinase levels are a prognostic indicator.

Basic Information

Product Name	Thymidine, Methyl-D3
CAS No.	74848-84-3
Molecular Formula	C10H11D3N2O5
Molecular Weight	245.23 g/mol
Synonyms	1-(2-Deoxy-β-D-ribofuranosyl)-5-methyl-d3-pyrimidine-2,4(1H,3H)-dione; [Methyl-d3]thymidine; Thymidine-methyl-d3; 2'-Deoxy-5-methyl-d3-uridine; dT-methyl-d3; 5-Methyl-d3-2'-deoxyuridine; Deuterated Thymidine (methyl-d3); Internal Standard for Thymidine
EINECS	Contact for details

Quality Control

Every batch of Thymidine, Methyl-D3 is manufactured under strict quality management systems and undergoes comprehensive analytical testing. We guarantee high chemical and isotopic purity, with specifications typically meeting or exceeding the requirements for sensitive mass spectrometric applications. A detailed Certificate of Analysis (COA), including data for assay, isotopic enrichment, and purity by HPLC and NMR, is provided with each shipment to ensure full traceability and compliance with your research or quality protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool place. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D (Methyl-d3)
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.