Description

Cytidine-2-14C Mol. Wt. 243.2 is a carbon-14 radiolabeled isotopologue of the essential nucleoside cytidine, specifically labeled at the 2-position of the pyrimidine ring. This compound is a critical tool for tracing metabolic pathways and studying nucleic acid synthesis, repair, and turnover in biochemical and pharmacological research. It is primarily used by researchers in pharmaceutical development, molecular biology, and metabolic studies who require precise tracking of nucleotide incorporation and metabolism.

Application

• Metabolic Pathway Tracing: Used as a radiotracer to study the de novo and salvage pathways of pyrimidine nucleotide biosynthesis and metabolism in cell cultures and animal models.
• Nucleic Acid Research: Essential for investigating DNA and RNA synthesis rates, repair mechanisms, and turnover kinetics in experimental systems.
• Drug Metabolism & Pharmacokinetics (DMPK): Employed in ADME (Absorption, Distribution, Metabolism, Excretion) studies to label and track the metabolic fate of nucleoside analog pharmaceuticals.
• Biochemical Assay Development: Serves as a key substrate or tracer in enzymatic assays for kinases, nucleotidyltransferases, and other nucleic acid-modifying enzymes.
• Cancer & Virology Research: Utilized to study the proliferation of cancer cells or viruses by monitoring the incorporation of labeled nucleosides into newly synthesized genetic material.
• Autoradiography & Imaging: Provides the radioactive source for autoradiographic techniques to visualize the spatial distribution of nucleotide uptake in tissues or gels.

Basic Information

Product Name	Cytidine-2-14C Mol. Wt. 243.2
CAS No.	74203-88-6
Molecular Formula	C9H1314CN3O5
Molecular Weight	243.2
Synonyms	2-14C-Cytidine; Cytidine-2-14C; 4-Amino-1-((2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)pyrimidin-2(1H)-one-2-14C; 1-β-D-Ribofuranosylcytosine-2-14C; [2-14C]Cytidine
EINECS	Contact for details

Quality Control

Every batch of our radiolabeled compounds is produced and tested to meet the stringent requirements of research applications. Radiochemical purity and specific activity are critically assessed using advanced analytical techniques such as HPLC with radiometric detection. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing the specific activity (mCi/mmol or GBq/mmol), radiochemical purity, chemical purity, and solvent composition. Our quality system ensures traceability and consistency for your long-term studies.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, solutions are recommended to be kept at -20°C in appropriate organic solvent (e.g., ethanol/water) to maintain stability and prevent radiolysis. Always follow local regulations for the safe handling and disposal of radioactive materials.

Specification

Item	Specification
Appearance	White to off-white solid or solution
Identification (HPLC, MS)	Conforms to reference standard
Specific Activity	50-60 mCi/mmol (1.85-2.22 GBq/mmol) [Typical]
Radiochemical Purity (HPLC)	≥ 97%
Chemical Purity (HPLC)	≥ 95%
Solvent (if supplied as solution)	Ethanol:Water (7:3 v/v) or specified

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.