Description

Hydrocortisone-[1,2,6,7-3H(N)] CAS NO 70094-60-9 is a high-specific-activity, tritium-labeled derivative of the endogenous glucocorticoid hormone hydrocortisone (cortisol). This radiochemical is an essential tool for advanced research requiring high sensitivity and specificity in detection and quantification. It is primarily used by scientists in pharmaceutical R&D, endocrinology, and molecular biology for applications such as receptor binding assays, metabolic studies, and tracer experiments in drug discovery. The product offers critical insights into glucocorticoid pathways and hormone action.

Application

• Receptor Binding Assays & Competitive Binding Studies: Used as a high-affinity radioligand to characterize glucocorticoid receptor (GR) density, affinity (Kd), and binding kinetics in vitro.
• Metabolic Pathway & Pharmacokinetic (PK) Research: Acts as a tracer to study the absorption, distribution, metabolism, and excretion (ADME) of hydrocortisone and related synthetic corticosteroids.
• Drug Discovery & Development Screening: Employed in high-throughput screening (HTS) platforms to identify and evaluate novel GR agonists, antagonists, and modulators.
• Endocrinology & Stress Response Research: Facilitates the study of hypothalamic-pituitary-adrenal (HPA) axis regulation, steroidogenesis, and the physiological effects of cortisol.
• Molecular Biology & Transactivation Studies: Used in cell-based reporter gene assays to investigate GR-mediated gene transcription and signal transduction pathways.
• Analytical Method Development & Validation: Serves as a critical reference standard in developing and validating sensitive analytical methods like LC-MS/MS or radio-HPLC.

Basic Information

Item	Detail
Product Name	Hydrocortisone-[1,2,6,7-3H(N)]
CAS No.	70094-60-9
Molecular Formula	C21H283H4O5
Molecular Weight	Contact for details
Synonyms	[1,2,6,7-3H]Cortisol; 11β,17α,21-Trihydroxy-4-pregnene-3,20-dione-[1,2,6,7-3H(N)]; Tritiated Hydrocortisone; [3H]-Hydrocortisone; [3H]-Cortisol; Cortisol-[1,2,6,7-3H(N)]; 4-Pregnene-11β,17α,21-triol-3,20-dione-[1,2,6,7-3H(N)]
EINECS	Contact for details

Quality Control

Every batch of Hydrocortisone-[1,2,6,7-3H(N)] is manufactured and handled under strict quality systems. It undergoes comprehensive analytical testing to ensure high radiochemical purity, specific activity, and chemical identity. Certificates of Analysis (COA) are provided, detailing results for key parameters including radiochemical purity (by HPLC), specific activity, and solvent composition. Our quality commitment aligns with the needs of GLP-compliant and research-grade laboratories.

Storage

Preserve in a tightly closed container, protected from light. Store at or below -20°C. This product is strictly light-sensitive (protect from all light exposure), highly volatile, and easily oxidized (store under inert atmosphere). For long-term stability, store in the original container within a designated, well-ventilated radioactive materials area. Allow the vial to equilibrate to room temperature before opening to minimize moisture ingress.

Specification

Item	Specification
Appearance	Clear, colorless to pale yellow solution
Identification (HPLC)	Conforms to reference standard
Radiochemical Purity (HPLC)	≥ 95%
Specific Activity	Contact for details (Typical range: 60-100 Ci/mmol)
Chemical Purity (HPLC)	≥ 98%
Solvent	Ethanol:Water (9:1) or as specified
Concentration	1 mCi/mL (or as specified)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.