Description

(-)-cis-Anti-N2-Bpde-Dg is a high-purity, stereochemically defined polycyclic aromatic hydrocarbon (PAH) metabolite and reference standard. This compound is critically important for toxicological research and analytical method development, serving as a key biomarker for DNA adduct formation studies. It is primarily utilized by research institutions, pharmaceutical companies, and contract testing laboratories engaged in carcinogenicity studies, metabolic pathway analysis, and environmental health safety (EHS) assessments.

Application

• Biomarker Reference Standard: For the quantification of specific DNA adducts in biological samples via advanced analytical techniques like 32P-postlabeling and LC-MS/MS.
• Toxicological Research: Fundamental studies on the mechanisms of carcinogenesis induced by benzo[a]pyrene and related polycyclic aromatic hydrocarbons (PAHs).
• Method Development & Validation: Serves as a critical calibrant and positive control in developing and validating sensitive assays for DNA damage detection.
• Environmental & Occupational Health: Used to assess exposure risks and biomonitoring in studies related to air pollution, tobacco smoke, and industrial processes.
• Pharmacokinetic/Pharmacodynamic (PK/PD) Studies: Investigation of the metabolic activation and detoxification pathways of procarcinogens.
• Quality Control in Testing Labs: Ensures accuracy and reliability in commercial and academic laboratories performing genotoxicity testing.

Basic Information

Product Name	(-)-cis-Anti-N2-Bpde-Dg
CAS No.	66113-73-3
Molecular Formula	C30H26N5O6P
Molecular Weight	583.53 g/mol
Synonyms	(-)-anti-BPDE-N2-dG; 7R,8S,9S,10R-7,8-Dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene 10-N2-deoxyguanosine adduct; anti-BPDE-dG Adduct; 10-(Deoxyguanosin-N2-yl)-7,8,9-trihydroxy-7,8,9,10-tetrahydrobenzo[a]pyrene; Benzo[a]pyrene diol epoxide deoxyguanosine adduct; BPDE-dG
EINECS	Contact for details

Quality Control

Our (-)-cis-Anti-N2-Bpde-Dg is manufactured under strict quality-controlled conditions suitable for research and analytical applications. Each batch is characterized and purity-verified using orthogonal techniques including HPLC, NMR, and MS to ensure identity, stereochemical purity, and chemical integrity. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and chromatographic data is provided with every shipment to support your regulatory and quality requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to prevent oxidation. Allow the sealed vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake and thermal stress. Store away from strong oxidizing agents.

Specification

Item	Specification
Appearance	Off-white to light tan solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95% (Area Percent)
Stereochemical Purity	≥ 98% (cis-anti isomer)
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.