Description

(+)-trans-Anti-Bpde-N2-Dg CAS NO 65437-20-9 is a high-purity, stereochemically defined benzo[a]pyrene diol epoxide (BPDE) derivative, specifically the (+)-trans-anti isomer covalently bound to deoxyguanosine (dG). This compound is of critical importance as a reference standard and research tool for studying the mechanisms of DNA damage and mutagenesis caused by polycyclic aromatic hydrocarbons (PAHs). It is primarily needed by academic, pharmaceutical, and toxicology research laboratories focused on carcinogenesis, biomarker development, and DNA adduct analysis.

Application

• Biomarker Research: Used as a definitive standard for the quantification of specific DNA adducts in biological samples via techniques like 32P-postlabeling, HPLC-fluorescence, or mass spectrometry.
• Mechanistic Toxicology Studies: Essential for investigating the metabolic activation of benzo[a]pyrene and the structural basis of its mutagenic and carcinogenic activity.
• Standards for Analytical Method Development: Serves as a critical calibrant and positive control in the development and validation of highly sensitive analytical methods for DNA damage detection.
• Cancer Research: Employed in studies to understand the relationship between specific DNA adduct formation, repair, and the initiation of cancer, particularly in models of lung and skin carcinogenesis.
• Quality Control in Reference Material Production: Used by manufacturers to certify and validate batches of related research chemicals and diagnostic kits.

Basic Information

Product Name	(+)-trans-Anti-Bpde-N2-Dg
CAS No.	65437-20-9
Molecular Formula	C31H27N5O5
Molecular Weight	549.58 g/mol
Synonyms	(+)-anti-BPDE-N2-dG; (+)-7β,8α-Dihydroxy-9α,10α-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene-N2-deoxyguanosine adduct; Benzo[a]pyrene-7,8-diol-9,10-epoxide-deoxyguanosine adduct (trans-anti isomer); BPDE-dG adduct; (+)-trans-BPDE-N2-dG; PAH-DNA adduct standard; B[a]PDE-N2-dG
EINECS	Contact for details

Quality Control

Our (+)-trans-Anti-Bpde-N2-Dg is produced and handled under strict quality protocols to ensure identity, purity, and stability for critical research applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing results from multiple orthogonal analytical techniques. We adhere to ISO 9001 quality management principles, and our documentation supports compliance with GLP (Good Laboratory Practice) requirements. Certificates of Analysis (COA) are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dedicated freezer. This compound is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Off-white to light tan solid
Identification (HPLC)	Retention time matches reference standard
Identification (MS)	Consistent with molecular ion [M+H]+
Purity (HPLC)	≥ 95% (Area % at 254 nm)
Isomeric Purity	≥ 98% (+)-trans-anti isomer
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.