Description

Glycocholic Acid-(Glycine-13C2) Mono-Hyd Rate, 99 Atom % 13C is a stable isotope-labeled analog of the primary bile acid, glycocholic acid, where the glycine moiety is enriched with two carbon-13 atoms. This high isotopic purity (99 atom % 13C) is critical for providing an internal standard with minimal isotopic interference in quantitative mass spectrometry. It is an essential tool for researchers and quality control laboratories in the pharmaceutical, clinical diagnostics, and metabolomics fields who require precise quantification of bile acids in complex biological matrices.

Application

• Internal Standard for LC-MS/MS: Used as a quantitative reference standard in liquid chromatography-tandem mass spectrometry (LC-MS/MS) assays for bile acid profiling in serum, plasma, and urine.
• Metabolomics Research: Enables accurate tracking and quantification of glycocholic acid and related metabolic pathways in studies of liver function, gut microbiome interactions, and metabolic disorders.
• Drug Development & Pharmacokinetics: Facilitates the precise measurement of bile acid concentrations in ADME (Absorption, Distribution, Metabolism, and Excretion) studies for drugs affecting hepatobiliary transport.
• Clinical Diagnostic Assays: Serves as a critical component in the development and validation of high-sensitivity diagnostic tests for cholestatic liver diseases, such as primary biliary cholangitis.
• Stable Isotope Dilution Analysis (SIDA): Provides the foundation for this gold-standard quantitative technique, ensuring high accuracy and reproducibility in analytical measurements.
• Biochemical Pathway Tracing: Allows for the detailed investigation of bile acid synthesis, conjugation, and enterohepatic circulation using isotope tracing methodologies.

Basic Information

Product Name	Glycocholic Acid-(Glycine-13C2) Mono-Hyd Rate, 99 Atom % 13C
CAS No.	63296-42-4
Molecular Formula	C26H4313C2NO7 • H2O
Molecular Weight	Approx. 489.6 g/mol (for 13C2 labeled form)
Synonyms	N-[(3α,5β,7α,12α)-3,7,12-Trihydroxy-24-oxocholan-24-yl]glycine-13C2 Monohydrate; Glycocholate-13C2; Taurocholic Acid Glycine-13C2 Analog; 3α,7α,12α-Trihydroxy-5β-cholan-24-oyl Glycine-13C2; GCA-13C2; Stable Isotope Labeled Glycocholic Acid; 13C2-Glycocholic Acid
EINECS	Contact for details

Quality Control

Every batch of our Glycocholic Acid-(Glycine-13C2) Mono-Hyd Rate is manufactured and analyzed under strict quality systems. The product undergoes rigorous testing to ensure it meets the high standards required for sensitive analytical applications, with a primary focus on isotopic purity and chemical identity. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing specifications for isotopic enrichment (99 atom % 13C), chemical purity, and identity confirmation.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. For short-term use, the product may be stored at 2-8°C. The material is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Isotopic Purity (13C)	≥ 99 atom %
Chemical Purity (HPLC)	≥ 98.0%
Water Content (KF)	3.0 - 7.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.