Description

n-Ethylmaleimide, [Ethyl-1-14C] is a carbon-14 labeled derivative of the classic sulfhydryl group alkylating agent, N-ethylmaleimide (NEM). This radiolabeled compound is essential for conducting precise metabolic and pharmacokinetic studies, enabling researchers to track the fate of molecules in biological systems with high sensitivity. It is a critical tool for scientists in pharmaceutical R&D, biochemistry, and molecular biology who require definitive data on protein modification, enzyme inhibition pathways, and drug metabolism.

Application

• Biochemical Research: As a site-specific, irreversible inhibitor for studying cysteine residues in enzymes and proteins.
• Metabolic Pathway Tracing: Radiolabel tracking of ethylmaleimide-derived adducts in in vitro and in vivo systems to elucidate biochemical pathways.
• Proteomics & Protein Chemistry: Mapping and quantifying reactive thiol groups in complex protein mixtures.
• Pharmacokinetic Studies: Used in ADME (Absorption, Distribution, Metabolism, Excretion) research for novel therapeutic agents that interact with thiol groups.
• Mechanism of Action Studies: Investigating the biochemical mechanisms of drugs and toxins that act via sulfhydryl modification.
• Reference Standard: Serves as an analytical standard in quantitative assays using techniques like Liquid Scintillation Counting (LSC) or accelerator mass spectrometry (AMS).

Basic Information

Product Name	n-Ethylmaleimide, [Ethyl-1-14C]
CAS No.	62356-37-0
Molecular Formula	C6H7NO2 (*C denotes 14C)
Molecular Weight	~126.13 g/mol (exact mass depends on 14C position)
Synonyms	1-Ethyl-1H-pyrrole-2,5-dione, [ethyl-1-14C]; N-Ethylmaleimide-1-14C; [1-14C]N-Ethylmaleimide; Radiolabeled NEM; 14C-NEM; NEM-14C; Maleic acid N-ethylimide-14C
EINECS	Contact for details

Quality Control

Every batch of our radiolabeled compounds is produced and analyzed under stringent conditions to ensure radiochemical purity, chemical purity, and specific activity meet the exacting requirements of research and regulatory submissions. Comprehensive quality testing includes HPLC with radiometric detection. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting the specific activity (mCi/mmol or GBq/mmol), radiochemical purity, chemical purity, and solvent composition.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at or below -20°C. The product should be kept in the original container in a designated, well-ventilated area for radioactive materials. Always follow local radiation safety regulations for handling and storage.

Specification

Item	Specification
Appearance	White to off-white solid or solution (as specified)
Identification (IR/NMR)	Conforms to structure
Specific Activity	50-60 mCi/mmol (1.85-2.22 GBq/mmol) [Typical]
Radiochemical Purity (HPLC)	≥ 97%
Chemical Purity (HPLC)	≥ 98%
Solvent (if supplied as solution)	Ethanol, Toluene, or other specified inert solvent
Concentration (if supplied as solution)	0.1 mCi/mL - 1.0 mCi/mL [Customizable]

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.