Description

2,4(1H,3H)-Pyrimidinedione, 5-(Bromomethyl)-, Labeled With Tritium (9Ci) is a high-specific-activity tritium-labeled nucleobase analog, essential for advanced biochemical and pharmacological research. This compound serves as a critical radioligand for tracing and quantifying biological pathways, offering unparalleled sensitivity in detection assays. It is primarily utilized by pharmaceutical R&D teams, contract research organizations (CROs), and academic institutions engaged in drug metabolism, pharmacokinetic (DMPK) studies, and molecular biology research.

Application

• Radioligand Binding Assays: Used as a high-affinity tracer for studying receptor-ligand interactions and enzyme kinetics.
• Drug Metabolism and Pharmacokinetics (DMPK): A vital tool for tracking the absorption, distribution, metabolism, and excretion (ADME) of novel pharmaceutical compounds.
• Biochemical Pathway Tracing: Enables precise monitoring of nucleotide incorporation and metabolic flux in cellular systems.
• Autoradiography and Imaging: Provides the radioactive source for high-resolution imaging studies in tissue sections.
• Proteomics and Biomarker Research: Facilitates the labeling and detection of biomolecules in complex mixtures.
• Quality Control for Radiopharmaceuticals: Serves as a reference standard in the development and validation of radioactive diagnostic agents.

Basic Information

Product Name	2,4(1H,3H)-Pyrimidinedione, 5-(Bromomethyl)-, Labeled With Tritium (9Ci)
CAS No.	61820-47-1
Molecular Formula	C5H5BrN2O2 (Tritium labeled)
Molecular Weight	Contact for details
Synonyms	5-(Bromomethyl)uracil-[3H]; [3H]-5-(Bromomethyl)uracil; 5-Bromomethyluracil, Tritiated; 5-(Bromomethyl)-2,4-dihydroxypyrimidine, Labeled With Tritium; 5-Bromo-1-methyluracil-[3H] (common misnomer); BrdUrd precursor (Tritiated); Tritium-labeled 5-Bromouracil derivative; Radiolabeled Uracil Analog
EINECS	Contact for details

Quality Control

Every batch of this radiolabeled compound is produced and analyzed under strict cGMP-like conditions to ensure identity, purity, and specific activity. Quality is verified through a battery of analytical techniques including HPLC with radiometric detection, NMR spectroscopy, and mass spectrometry. A comprehensive Certificate of Analysis (CoA) is provided with each shipment, detailing radionuclidic purity, chemical purity, specific activity (Ci/mmol), and solvent composition. Our quality systems are designed to meet the stringent requirements of FDA 21 CFR Part 211 for research materials.

Storage

Preserve in the original manufacturer's container as a solution in the specified solvent (typically ethanol or toluene). Store tightly sealed at -20°C or below. This product is strictly light-sensitive (protect from all light exposure) and may be moisture-sensitive. For long-term stability, store in a dedicated, well-ventilated freezer designated for radioactive materials, in accordance with local radiation safety regulations.

Specification

Item	Specification
Appearance	Clear, colorless to pale yellow solution
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to reference standard
Chemical Purity (HPLC)	≥ 98%
Radiochemical Purity	≥ 97%
Specific Activity	10-30 Ci/mmol (Contact for exact value)
Solvent	Ethanol or Toluene (Specified per batch)
Concentration	1.0 mCi/mL (Nominal)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.