Description

L-Isoleucine-15N is a stable isotope-labeled form of the essential amino acid L-Isoleucine, where the nitrogen atom is enriched with the 15N isotope. This precise labeling is critical for advanced research applications requiring high sensitivity and accurate metabolic tracing. It is an indispensable tool for scientists and manufacturers in the fields of nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry, metabolic pathway analysis, and the development of isotopically labeled pharmaceuticals and diagnostics.

Application

• Biomedical Research & NMR Spectroscopy: Used as an internal standard and tracer for studying protein structure, dynamics, and metabolic fluxes in complex biological systems.
• Quantitative Mass Spectrometry (MS): Serves as a critical standard for accurate quantification in proteomics and metabolomics studies.
• Isotope-Labeled Pharmaceutical Development: Employed in the synthesis of drug candidates and diagnostic agents for pharmacokinetic and pharmacodynamic studies.
• Nutritional & Metabolic Research: Used to investigate amino acid metabolism, protein synthesis rates, and nutritional status in clinical and preclinical models.
• Stable Isotope Labeling in Cell Culture (SILAC): A key component for heavy amino acid labeling in quantitative proteomics workflows.
• Environmental & Agricultural Science: Applied in studies of nitrogen cycling, plant metabolism, and soil microbial ecology.

Basic Information

Item	Detail
Product Name	L-Isoleucine-15N
CAS No.	59935-30-7
Molecular Formula	C₆H₁₃¹⁵NO₂
Molecular Weight	132.17 g/mol (approx.)
Synonyms	(2S,3S)-2-Amino-3-methylpentanoic acid-15N; L-2-Amino-3-methylvaleric acid-15N; (S)-Isoleucine-15N; ILE-15N; Iso-15N; 15N-L-Isoleucine; 15N-Ile; Stable Isotope Labeled L-Isoleucine
EINECS	Contact for details

Quality Control

Our L-Isoleucine-15N is manufactured under strict quality systems to ensure the highest isotopic and chemical purity for research applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing key parameters including isotopic enrichment (Atom % 15N), chemical purity, chiral purity, and specific tests for identity and impurities. We support custom synthesis to meet specific enrichment levels and packaging requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccated conditions or storage under an inert atmosphere to maintain stability and isotopic integrity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Isotopic Enrichment (Atom % 15N)	≥ 98%
Assay (HPLC)	≥ 98.0%
Chiral Purity (HPLC)	≥ 99.0% (L-isomer)
Specific Rotation	Contact for details
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.