Description

Guanosine 3',5'-Cyclic Phosphate, [Guanosine 8-3H] is a tritium-labeled derivative of cyclic guanosine monophosphate (cGMP), a crucial secondary messenger in cellular signal transduction pathways. This radiolabeled compound is essential for high-sensitivity detection and quantification in biochemical research, enabling precise tracking of cGMP dynamics. It is primarily used by researchers in pharmacology, molecular biology, and neuroscience for studying receptor-ligand interactions, enzyme activity assays, and intracellular signaling mechanisms.

Application

• Radioligand Binding Assays: Used to study the binding affinity and kinetics of cGMP to its target proteins, such as protein kinases, ion channels, and phosphodiesterases.
• Enzyme Activity Studies: Serves as a substrate or tracer in assays for guanylyl cyclase and phosphodiesterase activity, critical for drug discovery in cardiovascular and neurological disorders.
• Metabolic Pathway Tracing: Enables the investigation of cGMP synthesis, degradation, and downstream effector pathways in cell cultures and tissue samples.
• High-Throughput Screening (HTS): Employed in pharmaceutical R&D for screening compound libraries for modulators of the cGMP signaling pathway.
• Autoradiography & Imaging: Used in tissue section analysis to localize and visualize cGMP binding sites with high spatial resolution.
• Diagnostic Kit Development: Acts as a key component in the development of sensitive immunoassays and diagnostic tests for research purposes.

Basic Information

Product Name	Guanosine 3',5'-Cyclic Phosphate, [Guanosine 8-3H]
CAS No.	52898-16-5
Molecular Formula	C10H12N5O7P * 3H
Molecular Weight	~346.2 g/mol (unlabeled)
Synonyms	cGMP, [8-3H]; Cyclic GMP, Tritiated; 3',5'-Cyclic GMP, [8-3H]Guanosine; Guanosine 3',5'-cyclic monophosphate, tritium labeled; c[G(8-3H)MP]; [8-3H]-cGMP; Tritiated cyclic guanosine monophosphate; Cyclic 3',5'-guanosine monophosphate (tritiated)
EINECS	Contact for details

Quality Control

Our radiolabeled biochemicals are produced and handled under stringent quality protocols to ensure high specific activity, radiochemical purity, and chemical stability. Each batch is analyzed via HPLC and liquid scintillation counting to verify purity and concentration. A comprehensive Certificate of Analysis (COA) is provided, detailing specific activity, radiochemical purity, solvent composition, and analytical methods. We adhere to GLP (Good Laboratory Practice) standards to guarantee data integrity and product reliability for critical research applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at or below -20°C in the original solvent as supplied. Avoid repeated freeze-thaw cycles. Store in accordance with local regulations for radioactive materials.

Specification

Item	Specification
Appearance	Clear solution
Specific Activity	10-40 Ci/mmol (Contact for exact value)
Radiochemical Purity	≥ 95% (by HPLC)
Chemical Purity	≥ 98% (by HPLC, UV detection)
Concentration	1 mCi/mL (approx. 1.0 mCi in specified solvent)
Solvent	Ethanol:Water (7:3) or as specified
Identification	HPLC co-elution with reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.