Description

Naphthol As-Bi β-D-Galactopyranoside is a specialized chromogenic substrate used in enzymatic detection systems. This compound is critical for histochemical and cytochemical staining, where it serves as a precursor for localized color development upon enzymatic cleavage. It is primarily utilized by research institutions, diagnostic laboratories, and biotechnology companies engaged in molecular biology, pathology, and enzyme-linked assay development.

Application

• Histochemical Staining: Used as a substrate for β-galactosidase in tissue sections for localization of enzyme activity.
• Cytochemical Detection: Employed in cell-based assays to visualize β-galactosidase expression, commonly used in reporter gene studies.
• Diagnostic Kits: A key component in diagnostic kits for detecting specific bacterial enzymes, such as in microbiology identification systems.
• Biomedical Research: Facilitates the study of gene expression and cellular processes in developmental biology and neuroscience.
• Quality Control in Biotechnology: Used in enzymatic assays to monitor the activity of recombinant β-galactosidase enzymes during production.

Basic Information

Product Name	Naphthol As-Bi β-D-Galactopyranoside
CAS No.	51349-63-4
Molecular Formula	C23H24NO8
Molecular Weight	442.44 g/mol
Synonyms	6-Bromo-2-hydroxy-3-naphthoic acid 2-methoxy-4-(β-D-galactopyranosyloxy)anilide; Naphthol AS-BI β-D-galactopyranoside; Naphthol AS-BI-β-D-galactoside; AS-BI Galactoside; 3-Hydroxy-4-(β-D-galactopyranosyloxy)-2-naphthanilide; Galactopyranosyloxy Naphthol AS-BI; β-Galactosidase Substrate AS-BI
EINECS	Contact for details

Quality Control

Our Naphthol As-Bi β-D-Galactopyranoside is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, identity confirmation by IR and NMR spectroscopy, and residual solvent analysis. Certificates of Analysis (COA) detailing all specifications are provided to ensure compliance with research and diagnostic application requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is light-sensitive and should be handled under appropriate conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Meets ICH guidelines
Specific Rotation	Contact for details
Biological Activity	Suitable for histochemistry

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.