Description

Methoxyresorufin is a high-purity, synthetic chemical compound widely utilized as a critical substrate in biochemical research and diagnostic applications. Its primary value lies in its specific reactivity with cytochrome P450 enzymes, particularly CYP1A2, making it an indispensable tool for studying drug metabolism, enzyme induction, and inhibition. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and contract research organization (CRO) sectors who require reliable and consistent reagents for advanced metabolic studies.

Application

• Cytochrome P450 Enzyme Activity Assays: A selective fluorogenic substrate for measuring CYP1A2 isoform activity in microsomal preparations and recombinant enzyme systems.
• Drug Metabolism and Interaction Studies: Used in high-throughput screening (HTS) to evaluate the potential of new chemical entities to inhibit or induce specific metabolic pathways.
• Toxicological Research: Employed to assess the effects of environmental toxins, pharmaceuticals, and other xenobiotics on hepatic enzyme function.
• Diagnostic Kit Manufacturing: Serves as a key component in the formulation of in vitro diagnostic (IVD) kits for clinical and research use.
• Biomarker Development: Facilitates research into metabolic biomarkers by providing a precise method to quantify specific enzyme activities.
• Academic and Institutional Research: A standard reagent in university and government labs for fundamental research in pharmacology, biochemistry, and molecular biology.

Basic Information

Product Name	Methoxyresorufin
CAS No.	5725-89-3
Molecular Formula	C14H9NO4
Molecular Weight	255.23 g/mol
Synonyms	7-Methoxyresorufin; Methoxyresorufin; 3H-Phenoxazin-3-one, 7-methoxy-; Resorufin, 7-methoxy-; Benzoxazinone derivative; CYP1A2 substrate; EROD substrate (for Ethoxyresorufin-O-deethylase related assays)
EINECS	227-267-9

Quality Control

Every batch of Methoxyresorufin is manufactured and tested under a strict quality management system. We provide comprehensive analytical data to ensure the product meets the exacting standards required for sensitive enzymatic assays. A detailed Certificate of Analysis (COA) is supplied with each shipment, reporting key parameters such as identity, purity (by HPLC), and impurity profile. Our quality commitment supports compliance with relevant research and manufacturing guidelines.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	Red to dark red powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers
Functional Activity (CYP1A2)	Suitable for fluorometric enzyme activity assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.