Description

Uracil-15N2 is a stable isotope-labeled analog of the fundamental pyrimidine nucleobase, uracil, where two nitrogen atoms are enriched with the 15N isotope. This precise labeling makes it an indispensable internal standard and tracer in advanced mass spectrometry and NMR-based research, ensuring unparalleled accuracy in quantitative analysis. It is critically needed by researchers and manufacturers in the fields of pharmaceutical development, metabolomics, and stable isotope probing to study nucleic acid metabolism, drug mechanisms, and biological pathways.

Application

• Quantitative Mass Spectrometry: Serves as a critical internal standard for the precise quantification of uracil and related metabolites in complex biological matrices like plasma, urine, and cell lysates.
• NMR Spectroscopy Studies: Used as a tracer to elucidate metabolic pathways and flux analysis in vivo and in vitro, thanks to the distinct NMR signal of the 15N label.
• Pharmaceutical R&D: Employed in the development and validation of analytical methods for nucleoside/nucleotide-based drugs and in studying drug metabolism and pharmacokinetics (DMPK).
• Clinical Diagnostics Research: Facilitates the development of highly accurate LC-MS/MS assays for diagnosing inborn errors of metabolism related to pyrimidine catabolism.
• Microbial Ecology & SIP: Applied in Stable Isotope Probing (SIP) experiments to track nitrogen incorporation and identify active microorganisms in environmental samples.
• Biochemical Research: Essential for studying RNA synthesis, turnover, and the enzymatic mechanisms of kinases and synthases involved in nucleotide synthesis.

Basic Information

Product Name	Uracil-15N2
CAS No.	5522-55-4
Molecular Formula	C4H4N215N2O2
Molecular Weight	114.07 g/mol (for 15N2 labeled form)
Synonyms	2,4-Dihydroxypyrimidine-15N2; 2,4(1H,3H)-Pyrimidinedione-15N2; Uracil-1,3-15N2; [15N2]-Uracil; Isotopically labeled uracil; 15N2-Uracil; Pyrimidine-2,4-diol-15N2
EINECS	226-877-3 (for the unlabeled compound)

Quality Control

Our Uracil-15N2 is manufactured under strict quality systems to ensure the highest isotopic and chemical purity for research applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing isotopic enrichment (Atom % 15N), chemical purity, and identity confirmation. We adhere to relevant industry standards for stable isotope-labeled compounds, ensuring reliable and reproducible performance in your sensitive analytical workflows. Certificates of Analysis are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. The product is light-sensitive and should be handled accordingly to maintain stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (Atom % 15N)	≥ 98%
Chemical Purity (NMR)	Conforms
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.