Description

Resazurin is a vital redox indicator dye widely used in biochemical and microbiological assays. Its core value lies in its ability to serve as a visual and spectrophotometric marker for cell viability, bacterial metabolism, and sterility testing. This high-purity compound is essential for researchers and quality control professionals in the pharmaceutical, biotechnology, and food & beverage industries. Resazurin CAS NO 550-82-3 is a critical reagent for ensuring product safety and advancing scientific discovery.

Application

• Microbial Viability and Sterility Testing: A key component in resazurin-based assays (e.g., alamarBlue™) to measure bacterial and mammalian cell proliferation and metabolic activity.
• Milk Quality Control: Used in the standard resazurin reduction test to assess the microbiological quality and shelf-life of raw milk.
• Antimicrobial Susceptibility Testing (AST): Employed in rapid, colorimetric methods to determine the effectiveness of antibiotics against bacterial strains.
• Bioreactor and Fermentation Monitoring: Acts as an indicator for redox potential and metabolic activity in cell culture and fermentation processes.
• Toxicity and Cytotoxicity Screening: Utilized in in vitro assays to evaluate the cytotoxic effects of chemicals, drugs, or environmental samples.
• Dental Plaque Biofilm Assessment: Applied in dental research to quantify the metabolic activity of oral biofilms.
• Enzyme Activity Assays: Serves as a substrate or indicator in enzymatic reactions for research and diagnostic purposes.
• Educational and Research Laboratories: A fundamental dye for teaching and experimental protocols in biochemistry and microbiology.

Basic Information

Product Name	Resazurin
CAS No.	550-82-3
Molecular Formula	C12H7NO4
Molecular Weight	229.19 g/mol
Synonyms	Resazurin; 7-Hydroxy-3H-phenoxazin-3-one 10-oxide; Azoresorcin; Diazo-resorcinol; NSC 403697; EINECS 208-984-5; AlamarBlue™ indicator component
EINECS	208-984-5

Quality Control

Our Resazurin is manufactured under a strict quality management system to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including identification by FT-IR, purity assay by HPLC, and checks for relevant impurities. A Certificate of Analysis (COA) detailing all test results is provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled accordingly to maintain stability.

Specification

Item	Specification
Appearance	Dark red to brownish-red powder
Identification (IR)	Conforms to reference spectrum
Assay (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0 %
Resorufin (Related Substance)	≤ 1.0 %
Heavy Metals	≤ 20 ppm
Solubility (in Water)	Partially soluble; soluble in alkaline solution

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.