Description

Uridine-2-14C CAS NO 54-23-9 is a carbon-14 labeled isotopologue of the essential nucleoside uridine, specifically radiolabeled at the 2-position of the uracil base. This compound is a critical tool for tracing and quantifying metabolic pathways involving RNA synthesis, nucleotide salvage, and pyrimidine metabolism in biochemical and pharmacological research. It is primarily utilized by research scientists and process developers in the pharmaceutical, biotechnology, and life science sectors for advanced tracer studies, drug metabolism and pharmacokinetics (DMPK) investigations, and enzymatic assay development.

Application

• Drug Metabolism and Pharmacokinetics (DMPK) Studies: Used as a radiolabeled tracer to study the absorption, distribution, metabolism, and excretion (ADME) of novel pharmaceutical compounds, particularly those affecting nucleotide pathways.
• Nucleic Acid Metabolism Research: Essential for investigating the de novo and salvage pathways of pyrimidine biosynthesis and RNA turnover in cell culture and in vivo models.
• Enzymology and Assay Development: Serves as a specific substrate for assaying the activity of enzymes such as uridine kinase, uridine phosphorylase, and other nucleoside-metabolizing enzymes.
• Cancer and Virology Research: Employed in studies targeting rapidly dividing cells (e.g., cancer, viruses) to understand nucleotide utilization and the mechanism of action of antimetabolite drugs.
• Biochemical Pathway Tracing: Enables precise tracking of carbon flow in metabolic studies using techniques like LC-MS/MS or autoradiography to elucidate complex biological pathways.
• Standards and Calibration: Used as an internal standard in mass spectrometry for the accurate quantification of unlabeled uridine and its metabolites in biological samples.

Basic Information

Item	Detail
Product Name	Uridine-2-14C
CAS No.	54-23-9
Molecular Formula	C9H1214CN2O6
Molecular Weight	Contact for details
Synonyms	1-β-D-Ribofuranosyl-2-14C-uracil; Uridine-2-14C; [2-14C]Uridine; 2-14C-Labeled Uridine; Uridine (2-14C); β-Uridine-2-14C; NSC 20256-2-14C
EINECS	200-180-9

Quality Control

Every batch of Uridine-2-14C is manufactured and analyzed under strict quality systems to ensure the highest standards of radiochemical purity, chemical purity, and specific activity. Our quality commitment includes verification of identity and purity by advanced analytical techniques. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing batch-specific data including radiochemical purity (by HPLC), specific activity, and solvent composition. We support research compliance and data integrity for global markets.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage of solutions, aliquot and store under inert atmosphere at recommended temperatures. Always follow laboratory safety guidelines and local regulations for handling radioactive materials.

Specification

Item	Specification
Appearance	White to off-white solid or solution (as specified)
Identification (HPLC, MS)	Conforms to reference standard
Radiochemical Purity (HPLC)	≥ 97%
Chemical Purity (HPLC)	≥ 98%
Specific Activity	50-60 mCi/mmol (Contact for exact value)
Solvent (if supplied as solution)	Ethanol:Water (7:3) or as specified

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.