Description

2-[1,2-13C2,15N]Acetamido-2-Deoxy-D-[1-13C]Glucose is a stable isotope-labeled derivative of N-acetylglucosamine, a fundamental building block in glycobiology and metabolic research. This compound is essential for advanced tracer studies and quantitative mass spectrometry, enabling precise tracking of glycosylation pathways and carbohydrate metabolism. It is primarily utilized by research institutions and pharmaceutical companies engaged in drug discovery, biomarker validation, and the development of novel diagnostics.

Application

• Metabolic Flux Analysis (MFA): As a 13C and 15N-labeled tracer for studying the hexosamine biosynthesis pathway and glycosylation in cell cultures and animal models.
• Quantitative Proteomics & Glycomics: Used as an internal standard for the absolute quantification of N-acetylglucosamine and related glycoconjugates via LC-MS/MS.
• Pharmaceutical R&D: Investigation of metabolic disorders (e.g., diabetes), cancer metabolism, and the mode of action of glycosylation inhibitors.
• Biochemical Research: Substrate for enzymatic assays to study the kinetics of glycosyltransferases, hexosaminidases, and other carbohydrate-active enzymes.
• NMR Spectroscopy: Provides distinct 13C and 15N NMR signals for detailed structural and dynamic studies of carbohydrate-protein interactions.

Basic Information

Product Name	2-[1,2-13C2,15N]Acetamido-2-Deoxy-D-[1-13C]Glucose
CAS No.	478529-43-0
Molecular Formula	C813C2H1515NO6
Molecular Weight	~224.18 g/mol (for labeled form)
Synonyms	N-Acetyl-D-[1-13C]glucosamine-1,2-13C2,15N; [1-13C, 1,2-13C2,15N]-N-Acetylglucosamine; 2-Acetamido-2-deoxy-D-[1-13C]glucose-1,2-13C2,15N; Labeled GlcNAc; Isotopic N-Acetylglucosamine; 13C,15N-GlcNAc; NAG-13C,15N; (3R,4R,5S,6R)-5-acetamido-6-(hydroxymethyl)-3,4-dihydroxyoxane-2-carboxylic acid-13C,15N
EINECS	Contact for details

Quality Control

Every batch of our stable isotope-labeled compounds is produced and analyzed under strict quality protocols. We guarantee high chemical and isotopic purity verified by advanced analytical techniques including NMR, HPLC, and Mass Spectrometry. A comprehensive Certificate of Analysis (COA) detailing isotopic enrichment, chemical purity, and specific tests is provided with each shipment to ensure traceability and compliance with your research requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent moisture absorption.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98%
Isotopic Enrichment	≥ 99 atom % 13C; ≥ 98 atom % 15N
Chemical Purity (NMR/HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.