Description

2-[1,2-13C2]Acetamido-2-Deoxy-D-[Ul-13C6]Glucose is a highly enriched, stable isotope-labeled derivative of N-acetylglucosamine, a fundamental building block in glycobiology. This compound is specifically designed for advanced metabolic flux analysis and quantitative proteomics research, offering unparalleled precision for tracing biochemical pathways. It is an essential tool for researchers and analytical laboratories in the pharmaceutical, biotechnology, and life science sectors who require high-fidelity internal standards for mass spectrometry. The product is supplied with a CAS number of 478529-42-9.

Application

• Metabolic Flux Analysis (MFA): Used as a 13C-labeled tracer to study carbohydrate metabolism, glycosylation pathways, and energy production in cell cultures and biological systems.
• Quantitative Mass Spectrometry: Serves as a critical internal standard for the accurate quantification of N-acetylglucosamine and related metabolites in complex biological matrices via LC-MS or GC-MS.
• Glycobiology & Glycoprotein Research: Enables the study of O-GlcNAcylation dynamics, hexosamine biosynthetic pathway (HBP) activity, and the synthesis of labeled glycoconjugates.
• Pharmaceutical R&D: Facilitates drug metabolism and pharmacokinetics (DMPK) studies for compounds targeting metabolic disorders or glycosylation-related diseases.
• Biomarker Discovery: Employed in stable isotope labeling by amino acids in cell culture (SILAC) and other labeling techniques to identify and validate disease biomarkers.
• Nuclear Magnetic Resonance (NMR) Spectroscopy: Provides a highly sensitive probe for structural and dynamic studies of biomolecules due to its uniform 13C labeling pattern.

Basic Information

Item	Details
Product Name	2-[1,2-13C2]Acetamido-2-Deoxy-D-[Ul-13C6]Glucose
CAS No.	478529-42-9
Molecular Formula	C813C2H15NO6
Molecular Weight	~227.18 (for 13C8 labeled form)
Synonyms	N-Acetyl-D-[Ul-13C6]glucosamine-1,2-13C2; [1,2-13C2, Ul-13C6]-N-Acetylglucosamine; 13C8-Labeled N-Acetylglucosamine; 2-Acetamido-2-deoxy-D-[13C6]glucose-1,2-13C2; GlcNAc-13C8; NAG-13C8; Stable Isotope Labeled N-Acetyl-D-glucosamine; Per-13C-labeled N-Acetylglucosamine
EINECS	Contact for details

Quality Control

Every batch of our stable isotope-labeled compounds is produced and analyzed under stringent quality protocols to ensure isotopic and chemical purity for sensitive research applications. We provide comprehensive Certificates of Analysis (COA) detailing isotopic enrichment (typically >99 atom% 13C), chemical purity (by HPLC), and identity confirmation (by NMR and MS). Our quality system is designed to meet the exacting standards of analytical and pharmaceutical research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, containers should be sealed under an inert atmosphere and kept in a desiccator to maintain stability and isotopic integrity. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Isotopic Enrichment	≥99 atom% 13C
Chemical Purity (HPLC)	≥98%
Specific Rotation	Contact for details
Water Content (KF)	<1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.