Description

[5',5''-2H2]Guanosine Monohydrate is a stable isotope-labeled nucleoside derivative, specifically deuterated at the 5' and 5'' positions of the ribose sugar. This compound is essential for advanced analytical research, providing an internal standard for the precise quantification of guanosine and related metabolites via mass spectrometry. It is primarily utilized by researchers and manufacturers in pharmaceutical development, metabolomics, and clinical diagnostics who require high-fidelity isotopic purity for accurate and reproducible results.

Application

• Mass Spectrometry Internal Standard: Used as a quantitative reference standard in LC-MS/MS and GC-MS analyses for guanosine and its analogs in biological matrices.
• Metabolomics Research: Enables the tracing and quantification of nucleotide metabolism and purine salvage pathways in cell culture and in vivo studies.
• Pharmaceutical R&D: Supports drug metabolism and pharmacokinetics (DMPK) studies for nucleoside-based therapeutics, including antivirals and anticancer agents.
• Clinical Diagnostics: Facilitates the development of high-precision assays for measuring guanosine levels, which can be biomarkers for certain physiological conditions.
• Isotope Dilution Analysis: Critical for achieving high accuracy and minimizing matrix effects in complex sample analysis.
• Biochemical Reagent: Serves as a labeled precursor in enzymatic studies to investigate RNA synthesis and processing mechanisms.

Basic Information

Product Name	[5',5''-2H2]Guanosine Monohydrate
CAS No.	478511-34-1
Molecular Formula	C10H11D2N5O5 • H2O
Molecular Weight	301.27 g/mol (for monohydrate)
Synonyms	Deuterated Guanosine Monohydrate; Guanosine-5',5''-d2 Monohydrate; 9-β-D-Ribofuranosylguanine-5',5''-d2 Monohydrate; 2-Amino-1,9-dihydro-9-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl-d2)oxolan-2-yl]-6H-purin-6-one Monohydrate; [5',5''-2H2]-Guanosine Monohydrate; Guanosine-d2 (5',5'') Monohydrate
EINECS	Contact for details

Quality Control

Every batch of [5',5''-2H2]Guanosine Monohydrate is manufactured and tested under strict quality systems. We guarantee high isotopic and chemical purity, with specifications typically meeting or exceeding the requirements for research-grade labeled compounds. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing parameters such as isotopic purity (D-enrichment), chemical purity (by HPLC), and identity confirmation (by NMR/MS).

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation. Keep container tightly sealed when not in use to prevent absorption of atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-enrichment)	≥ 98 atom % D
Water Content (KF)	5.0 - 8.0% (Theoretical for monohydrate: ~6.0%)
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.