Description

[5'-13C]Uridine is a stable isotope-labeled nucleoside where the carbon-13 isotope is specifically incorporated at the 5' position of the ribose sugar. This precise labeling is critical for advanced metabolic research, enabling the accurate tracing of RNA synthesis and nucleotide metabolism pathways in biological systems. It is an essential tool for researchers and developers in pharmaceutical R&D, clinical diagnostics, and academic biochemistry who require high-purity, isotopically defined standards for mass spectrometry and NMR studies.

Application

• Metabolic Flux Analysis (MFA): Used as a tracer to quantify the rates of metabolic pathways, particularly in studies of pyrimidine and nucleotide synthesis.
• Mass Spectrometry (MS) Internal Standard: Serves as a critical quantitative standard in LC-MS/MS and GC-MS methods for accurate measurement of endogenous uridine levels in biological samples.
• Nuclear Magnetic Resonance (NMR) Spectroscopy: Provides a non-radioactive, stable isotope probe for structural and dynamic studies of RNA and nucleoside interactions.
• Pharmaceutical Development: Employed in drug metabolism and pharmacokinetics (DMPK) studies to track the incorporation of nucleoside analogs into RNA.
• Clinical Research: Facilitates investigations into disorders of nucleotide metabolism and the mechanisms of antiviral or anticancer nucleoside drugs.
• Biochemical Pathway Elucidation: Enables detailed mapping of the de novo and salvage pathways of uridine metabolism in cell cultures and model organisms.

Basic Information

Product Name	[5'-13C]Uridine
CAS No.	478511-16-9
Molecular Formula	C9H1213CN2O6
Molecular Weight	Contact for details
Synonyms	Uridine-5'-13C; 1-β-D-Ribofuranosyluracil-5'-13C; 5'-13C-Labeled Uridine; Uridine (5'-13C); Stable Isotope Labeled Uridine; [5'-13C]-Urd; [13C]Uridine (position-specific); Isotopic Uridine Standard
EINECS	Contact for details

Quality Control

Every batch of [5'-13C]Uridine is manufactured and analyzed under strict quality protocols to ensure exceptional isotopic and chemical purity, which is paramount for sensitive analytical applications. Our products undergo rigorous quality testing, including advanced spectroscopic and chromatographic methods, to ensure compliance with the exacting standards required for research and development. Certificates of Analysis (COA) detailing isotopic enrichment (atom % 13C), chemical purity, and analytical methods are provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C (refrigerated) or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation and storage under inert atmosphere to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 99 atom % 13C (5' position)
Isotopic Purity (MS)	≥ 99%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.